Genomic and Metabolic Diversity of Marine Group I Thaumarchaeota in the Mesopelagic of Two Subtropical Gyres
| العنوان: | Genomic and Metabolic Diversity of Marine Group I Thaumarchaeota in the Mesopelagic of Two Subtropical Gyres |
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| المؤلفون: | E. Dashiell P. Masland, Nicole J. Poulton, Erin K. Field, Manuel Martinez-Garcia, Patrick S. G. Chain, Alexander Sczyrba, Brandon K. Swan, Tanja Woyke, Hilary G. Morrison, Ramunas Stepanauskas, Christopher C. Harris, Sergey Koren, Mark Chaffin |
| المساهمون: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Ecología Microbiana Molecular |
| المصدر: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) PLoS ONE PLoS ONE, Vol 9, Iss 4, p e95380 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Thaumarchaeota, Mesopelagic zone, Nitrosopumilus, lcsh:Medicine, Chemoautotrophs, Cenarchaeum symbiosum, Microbiología, Microbiology, Microbial Ecology, 03 medical and health sciences, Phylogenetics, Microbial Physiology, Genetics, 14. Life underwater, Genome Sequencing, Archaean Biology, lcsh:Science, Molecular Biology Techniques, Sequencing Techniques, Molecular Biology, Marine Group I Thaumarchaeota, Phylogeny, 030304 developmental biology, Microbial Metabolism, 0303 health sciences, Multidisciplinary, biology, Phylogenetic tree, Ecology, 030306 microbiology, lcsh:R, Biology and Life Sciences, Computational Biology, Subtropical, Genomics, Genomic and metabolic diversity, biology.organism_classification, Archaea, DNA, Archaeal, Evolutionary biology, Metagenomics, lcsh:Q, Research Article |
| الوصف: | Marine Group I (MGI) Thaumarchaeota are one of the most abundant and cosmopolitan chemoautotrophs within the global dark ocean. To date, no representatives of this archaeal group retrieved from the dark ocean have been successfully cultured. We used single cell genomics to investigate the genomic and metabolic diversity of thaumarchaea within the mesopelagic of the subtropical North Pacific and South Atlantic Ocean. Phylogenetic and metagenomic recruitment analysis revealed that MGI single amplified genomes (SAGs) are genetically and biogeographically distinct from existing thaumarchaea cultures obtained from surface waters. Confirming prior studies, we found genes encoding proteins for aerobic ammonia oxidation and the hydrolysis of urea, which may be used for energy production, as well as genes involved in 3-hydroxypropionate/4-hydroxybutyrate and oxidative tricarboxylic acid pathways. A large proportion of protein sequences identified in MGI SAGs were absent in the marine cultures Cenarchaeum symbiosum and Nitrosopumilus maritimus, thus expanding the predicted protein space for this archaeal group. Identifiable genes located on genomic islands with low metagenome recruitment capacity were enriched in cellular defense functions, likely in response to viral infections or grazing. We show that MGI Thaumarchaeota in the dark ocean may have more flexibility in potential energy sources and adaptations to biotic interactions than the existing, surface-ocean cultures. This work was supported by NSF grants EF-826924 (R.S.), OCE-821374 (R.S.) and OCE-1232982 (R.S. and B.K.S.); the DOE JGI 2010 Microbes Program grant CSP77 (R.S. and M.E.S.); National Institutes of Health grant 1UH2DK083993 (H.G.M.). Work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The contributions of S.K. were funded under Agreement No. HSHQDC-07-C-00020 awarded by the Department of Homeland Security (DHS) for the management and operation of the National Biodefense Analysis and Countermeasures Center (NBACC), a Federally Funded Research and Development Center. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69f0933c9e526d0c0f2eac34b18a5960 http://hdl.handle.net/10045/36812 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....69f0933c9e526d0c0f2eac34b18a5960 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |