Chloroplast Outer Membrane β-Barrel Proteins Use Components of the General Import Apparatus
| العنوان: | Chloroplast Outer Membrane β-Barrel Proteins Use Components of the General Import Apparatus |
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| المؤلفون: | Philip M. Day, Kentaro Inoue, Steven M. Theg |
| المصدر: | The Plant Cell. 31:1845-1855 |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Chloroplasts, Arabidopsis, Nicotiana benthamiana, Plant Science, Biology, 01 natural sciences, Chloroplast membrane, In Brief, Chloroplast Proteins, 03 medical and health sciences, Transit Peptide, Tobacco, Plant Proteins, Arabidopsis Proteins, Membrane Proteins, food and beverages, Cell Biology, Chloroplast outer membrane, biology.organism_classification, Transmembrane protein, Cell biology, Chloroplast, Protein Transport, 030104 developmental biology, Membrane, 010606 plant biology & botany |
| الوصف: | Chloroplasts evolved from a cyanobacterial endosymbiont that resided within a eukaryotic cell. Due to their prokaryotic heritage, chloroplast outer membranes contain transmembrane β-barrel proteins. While most chloroplast proteins use N-terminal transit peptides to enter the chloroplasts through the translocons at the outer and inner chloroplast envelope membranes (TOC/TIC), only one β-barrel protein, Toc75, has been shown to use this pathway. The route other β-barrel proteins use has remained unresolved. Here we use in vitro pea (Pisum sativum) chloroplast import assays and transient expression in Nicotiana benthamiana to address this. We show that a paralog of Toc75, outer envelope protein 80 kD (OEP80), also uses a transit peptide but has a distinct envelope sorting signal. Our results additionally indicate that β-barrels that do not use transit peptides also enter the chloroplast using components of the general import pathway. |
| تدمد: | 1532-298X 1040-4651 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a254aef4bb5ac67f6f79de3c9f42a3a https://doi.org/10.1105/tpc.19.00001 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6a254aef4bb5ac67f6f79de3c9f42a3a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1532298X 10404651 |
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