Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana
| العنوان: | Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana |
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| المؤلفون: | Jie-Xia Liu, Zhi-Sheng Xu, Ai-Sheng Xiong, Tong Li, Yuan-Jie Deng |
| المصدر: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-13 (2021) BMC Plant Biology |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lutein, Drought stress, Abscisic acid synthesis, Transgene, Drought tolerance, Arabidopsis, Gene Expression, Taproot, Plant Science, Antioxidants, Mixed Function Oxygenases, chemistry.chemical_compound, Plant Growth Regulators, Gene Expression Regulation, Plant, Stress, Physiological, Carrot, Arabidopsis thaliana, Carotenoid, Abscisic acid, Plant Proteins, chemistry.chemical_classification, β-Carotene hydroxylase, biology, Research, fungi, Botany, food and beverages, ROS, biology.organism_classification, Plants, Genetically Modified, Carotenoids, Daucus carota, Droughts, Horticulture, chemistry, QK1-989, Abscisic Acid |
| الوصف: | Background Carrot (Daucus carota L.), an important root vegetable, is very popular among consumers as its taproot is rich in various nutrients. Abiotic stresses, such as drought, salt, and low temperature, are the main factors that restrict the growth and development of carrots. Non-heme carotene hydroxylase (BCH) is a key regulatory enzyme in the β-branch of the carotenoid biosynthesis pathway, upstream of the abscisic acid (ABA) synthesis pathway. Results In this study, we characterized a carrot BCH encoding gene, DcBCH1. The expression of DcBCH1 was induced by drought treatment. The overexpression of DcBCH1 in Arabidopsis thaliana resulted in enhanced tolerance to drought, as demonstrated by higher antioxidant capacity and lower malondialdehyde content after drought treatment. Under drought stress, the endogenous ABA level in transgenic A. thaliana was higher than that in wild-type (WT) plants. Additionally, the contents of lutein and β-carotene in transgenic A. thaliana were lower than those in WT, whereas the expression levels of most endogenous carotenogenic genes were significantly increased after drought treatment. Conclusions DcBCH1 can increase the antioxidant capacity and promote endogenous ABA levels of plants by regulating the synthesis rate of carotenoids, thereby regulating the drought resistance of plants. These results will help to provide potential candidate genes for plant drought tolerance breeding. |
| اللغة: | English |
| تدمد: | 1471-2229 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a2ec3d498efb18bb1961d16e643c312 https://doaj.org/article/6468cd13141f43d28a57d1057429082b |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6a2ec3d498efb18bb1961d16e643c312 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712229 |
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