Subnormal GM1 in PBMCs: Promise for Early Diagnosis of Parkinson's Disease?
| العنوان: | Subnormal GM1 in PBMCs: Promise for Early Diagnosis of Parkinson's Disease? |
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| المؤلفون: | Suman Chowdhury, Robert W. Ledeen, Roy N. Alcalay, Monami Chakraborty, Matthew Surface, Samar K. Alselehdar |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 11522, p 11522 (2021) International Journal of Molecular Sciences Volume 22 Issue 21 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | GBA variant of Parkinson’s, Male, Parkinson's disease, Disease, medicine.disease_cause, HPTLC, GD1a ganglioside, Gangliosides, Biology (General), sporadic Parkinson’s disease, Spectroscopy, Cholera toxin, Parkinson Disease, General Medicine, Middle Aged, Computer Science Applications, Gm1 ganglioside, Fully developed, Chemistry, Glucosylceramidase, Female, QH301-705.5, G(M1) Ganglioside, Peripheral blood mononuclear cell, Article, Catalysis, Inorganic Chemistry, medicine, Humans, Physical and Theoretical Chemistry, cholera toxin B, Molecular Biology, QD1-999, Aged, business.industry, Organic Chemistry, medicine.disease, Future study, Early Diagnosis, ROC Curve, Case-Control Studies, Immunology, PBMCs, Mutation, Leukocytes, Mononuclear, GM1 ganglioside, business, Glucocerebrosidase, Biomarkers, Blood Chemical Analysis |
| الوصف: | The fact that Parkinson’s disease (PD) pathologies are well advanced in most PD patients by the time of clinical elucidation attests to the importance of early diagnosis. Our attempt to achieve this has capitalized on our previous finding that GM1 ganglioside is expressed at subnormal levels in virtually all tissues of sporadic PD (sPD) patients including blood cells. GM1 is present in most vertebrate cells, is especially abundant in neurons where it was shown essential for their effective functioning and long term viability. We have utilized peripheral blood mononuclear cells (PBMCs) which, despite their low GM1, we found to be significantly lower in sPD patients compared to age-matched healthy controls. To quantify GM1 (and GD1a) we used high performance thin-layer chromatography combined with cholera toxin B linked to horseradish peroxidase, followed by densitometric quantification. GM1 was also deficient in PBMCs from PD patients with mutations in the glucocerebrosidase gene (PD-GBA), apparently even lower than in sPD. Reasons are given why we believe these results obtained with patients manifesting fully developed PD will apply as well to PD patients in preclinical stages—a topic for future study. We also suggest that these findings point to a potential disease altering therapy for PD once the early diagnosis is established. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ab5c9a01807861304e0f14d288e38c3 https://pubmed.ncbi.nlm.nih.gov/34768952 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6ab5c9a01807861304e0f14d288e38c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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