Inhibitory effect of STAT3 gene combined with CDDP on growth of human Wilms tumour SK-NEP-1 cells
| العنوان: | Inhibitory effect of STAT3 gene combined with CDDP on growth of human Wilms tumour SK-NEP-1 cells |
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| المؤلفون: | Junhui Yuan, Junrong Wang, Haijiang Qu, Guangxian You, Caie Chen, Wenyi Li, Nina Zhang, Feng Pan |
| المصدر: | Bioscience Reports |
| بيانات النشر: | Portland Press Ltd., 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | STAT3 Transcription Factor, 0301 basic medicine, Cell, Biophysics, S48, S42, Wilms Tumor, Biochemistry, STAT3, Mice, 03 medical and health sciences, 0302 clinical medicine, Bcl-2-associated X protein, Cell Line, Tumor, Heat shock protein, medicine, Animals, Humans, Endoplasmic Reticulum Chaperone BiP, Molecular Biology, Heat-Shock Proteins, bcl-2-Associated X Protein, Cisplatin, Original Paper, cisplatin (CDDP), glucose regulatory protein 78 (GRP78), biology, Wilms tumour (WT), Wilms' tumor, Cell Biology, medicine.disease, Original Papers, Xenograft Model Antitumor Assays, Molecular biology, Gene Expression Regulation, Neoplastic, 030104 developmental biology, medicine.anatomical_structure, S39, BAX, 030220 oncology & carcinogenesis, biology.protein, Cancer research, STAT protein, Immunohistochemistry, medicine.drug |
| الوصف: | To investigate the effects of signal transducer and activator of transcription 3 (STAT3) combined with cisplatin (CDDP) on the growth of human Wilms tumour (WT) SK-NEP-1 cell subcutaneous xenografts in nude mice and the possible mechanisms. Human WT SK-NEP-1 cells were subcutaneously transplanted to establish the BALB/c nude mice xenograft model. Mice were randomly divided into five groups: blank control group, adenovirus control group (NC group), STAT3 group, CDDP group and STAT3 plus CDDP group (combination group). Tumour volume and tumour weight were observed during the therapeutic process. The expression levels of STAT3, glucose regulatory protein 78 (GRP78) and BCL2-associated X protein (BAX) were evaluated by immunohistochemical analysis. Compared with the STAT3 group or CDDP group, the tumour weight and volume was significantly reduced in the combination group (P 0.05). Immunohistochemical analysis showed that STAT3, GRP78 and BAX protein levels in the combination group were significantly higher than those in STAT3 group and CDDP group (P |
| تدمد: | 1573-4935 0144-8463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6abd28892b10431346f728adee31e88a https://doi.org/10.1042/bsr20160072 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6abd28892b10431346f728adee31e88a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734935 01448463 |
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