Effects of chronic metal exposure on wild fish populations revealed by high-throughput cDNA sequencing
| العنوان: | Effects of chronic metal exposure on wild fish populations revealed by high-throughput cDNA sequencing |
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| المؤلفون: | Peter G. C. Campbell, Patrice Couture, Fabien Pierron, Louis Bernatchez, Michel Amery Defo, Eric Normandeau |
| المصدر: | Ecotoxicology. 20:1388-1399 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Fish Proteins, DNA, Complementary, Health, Toxicology and Mutagenesis, Gene Expression, Management, Monitoring, Policy and Law, Biology, Toxicology, DNA sequencing, Bile Acids and Salts, Transcriptome, Transcription (biology), Protein biosynthesis, Animals, Gene silencing, Epigenetics, Vitamin A, Gene, Genetics, Gene Expression Profiling, Fishes, High-Throughput Nucleotide Sequencing, General Medicine, Metals, Pyrosequencing, Copper, Water Pollutants, Chemical, Cadmium |
| الوصف: | Given the inherent variability of aquatic systems, predicting the in situ effects of contaminants on such ecosystems still represents a major challenge for ecotoxicology. In this context, transcriptomic tools can help identify and investigate the mechanisms of toxicity beyond the traditional morphometric, physiological and population-level endpoints. In this study, we used the 454 sequencing technology to examine the in situ effects of chronic metal (Cd, Cu) exposure on the yellow perch (Perca flavescens) transcriptome. Total hepatic mRNA from fish sampled along a polymetallic gradient was extracted, reverse transcribed, labeled with unique barcode sequences and sequenced. This approach allowed us to identify correlations between the transcription level of single genes and the hepatic concentrations of individual metals; 71% of the correlations established were negative. Chronic metal exposure was thus associated with a decrease in the transcription levels of numerous genes involved in protein biosynthesis, in the immune system, and in lipid and energy metabolism. Our results suggest that this marked decrease could result from an impairment of bile acid metabolism by Cd and energy restriction but also from the recruitment of several genes involved in epigenetic modifications of histones and DNA that lead to gene silencing. |
| تدمد: | 1573-3017 0963-9292 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ba5b87bf1e2626f17e1e432780205ce https://doi.org/10.1007/s10646-011-0696-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6ba5b87bf1e2626f17e1e432780205ce |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15733017 09639292 |
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