Astragaloside positively regulated osteogenic differentiation of pre-osteoblast MC3T3-E1 through PI3K/Akt signaling pathway

التفاصيل البيبلوغرافية
العنوان: Astragaloside positively regulated osteogenic differentiation of pre-osteoblast MC3T3-E1 through PI3K/Akt signaling pathway
المؤلفون: Wei Bing Jing, Hongjuan Ji, Jinlong Wang, Rui Jiang
المصدر: Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-10 (2021)
Journal of Orthopaedic Surgery and Research
بيانات النشر: BMC, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Diseases of the musculoskeletal system, chemistry.chemical_compound, Phosphatidylinositol 3-Kinases, Astragaloside, Western blot, stomatognathic system, Osteogenesis, medicine, Humans, Orthopedics and Sports Medicine, LY294002, Viability assay, MC3T3-E1 cells, PI3K/AKT/mTOR pathway, Orthopedic surgery, Osteoblasts, medicine.diagnostic_test, Akt/PKB signaling pathway, business.industry, Osteoblast, Cell Differentiation, Mc3t3 e1, Molecular biology, medicine.anatomical_structure, chemistry, RC925-935, PI3K/Akt pathway, Osteoporosis, Surgery, business, Proto-Oncogene Proteins c-akt, RD701-811, Research Article, Signal Transduction
الوصف: Background Osteoporosis is a widespread chronic disease characterized by low bone density. There is currently no gold standard treatment for osteoporosis. The aim of this study was to explore the role and mechanism of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. Methods MC3T3-E1 cells were divided into control and different dose of Astragaloside (10, 20, 40, 50, and 60 μg/ml). Then, ALP and ARS staining were performed to identify the effects of Astragaloside for early and late osteogenic capacity of MC3T3-E1 cells, respectively. Real-time PCR and western blot were performed to assess the ALP, OCN, and OSX expression. PI3K/Akt signaling pathway molecules were then assessed by Western blot. Finally, PI3K inhibitor, LY294002, was implemented to assess the mechanism of Astragaloside in promoting osteogenic differentiation of MC3T3-E1 cells. Results Astragaloside significantly increased the cell viability than the control group. Moreover, Astragaloside enhanced the ALP activity and calcium deposition than the control groups. Compared with the control group, Astragaloside increased the ALP, OCN, and OSX expression in a dose-response manner. Western blot assay further confirmed the real-time PCR results. Astragaloside could significantly increase the p-PI3K and p-Akt expression than the control group. LY294002 partially reversed the promotion effects of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. LY294002 partially reversed the promotion effects of Astragaloside on ALP, OCN, and OSX of MC3T3-E1 cells. Conclusion The present study suggested that Astragaloside promoted osteogenic differentiation of MC3T3-E1 cells through regulating PI3K/Akt signaling pathway.
اللغة: English
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bdf9ca10d9d240bdc578016459ca4f7
https://doaj.org/article/4cf47ff3221843e5bb7d483c2113d7c9
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....6bdf9ca10d9d240bdc578016459ca4f7
قاعدة البيانات: OpenAIRE