Detection and Characterization of Small Midregion Parathyroid Hormone Fragment(s) in Normal and Hyperparathyroid Glands and Sera by Immunoextraction and Region-Specific Radioimmunoassays*
| العنوان: | Detection and Characterization of Small Midregion Parathyroid Hormone Fragment(s) in Normal and Hyperparathyroid Glands and Sera by Immunoextraction and Region-Specific Radioimmunoassays* |
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| المؤلفون: | Joyce Ells, M. Yoon, David C. Aron, J. Pensky, M. B. Huber, Arnold W. Lindall, Bernard A. Roos, J. W. Orf, Phillip W. Lambert |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 53:709-721 |
| بيانات النشر: | The Endocrine Society, 1981. |
| سنة النشر: | 1981 |
| مصطلحات موضوعية: | Adult, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Radioimmunoassay, Parathyroid hormone, complex mixtures, Biochemistry, Parathyroid Glands, fluids and secretions, Endocrinology, Region specific, Internal medicine, Mole, medicine, Humans, Edetate disodium, Immunosorbent Techniques, Aged, Chemistry, Hyperparathyroidism, Biochemistry (medical), Cross reactions, Middle Aged, Intact pth, medicine.disease, Molecular Weight, Parathyroid Hormone, Chromatography, Gel, Calcium, hormones, hormone substitutes, and hormone antagonists, Primary hyperparathyroidism |
| الوصف: | We have employed region-specific RIAs directed against amino-terminal (N), midregion (M), or carboxyl-terminal (C) regions of parathyroid hormone (PTH) to analyze glandular and circulating PTH species in normal subjects and in persons with primary hyperparathyroidism. Immunoaffinity methods have been developed for validation of RIA measurements and for extraction of circulating PTH species. Chromatographic analyses of normal and adenomatous parathyroid-gland extracts and electrophoretic analyses of parathyroid-gland immunoextracts revealed at least four PTH species, each with a distinct pattern of cross-reactivity in the sequence-specific RIAs. Intact PTH (mol wt, 9500) was detected by each type of RIA. The large carboxyl-fragment (mol wt, 5500), was detected only by M and C RIAs. A somewhat smaller fragment (mol wt, 4000) was identified that was detected by M RIA but not by C RIA. We also detected in parathyroid extracts a small N-fragment (mol wt, 3400), which had no reactivity in M or C RIAs. In normal... |
| تدمد: | 1945-7197 0021-972X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bdfffd61a847cecf3534b68ac35e0a1 https://doi.org/10.1210/jcem-53-4-709 |
| رقم الأكسشن: | edsair.doi.dedup.....6bdfffd61a847cecf3534b68ac35e0a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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