High potassium promotes mutual interaction between (pro)renin receptor and the local renin-angiotensin-aldosterone system in rat inner medullary collecting duct cells
| العنوان: | High potassium promotes mutual interaction between (pro)renin receptor and the local renin-angiotensin-aldosterone system in rat inner medullary collecting duct cells |
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| المؤلفون: | Aihua Lu, Li Zhou, Chuanming Xu, Tianxin Yang, Hui Fang |
| المصدر: | American Journal of Physiology-Cell Physiology. 311:C686-C695 |
| بيانات النشر: | American Physiological Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Medullary cavity, Physiology, Potassium, Pro renin receptor, chemistry.chemical_element, Receptors, Cell Surface, 030204 cardiovascular system & hematology, Losartan, Rats, Sprague-Dawley, Renin-Angiotensin System, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Renin, Renin–angiotensin system, medicine, Animals, Cytochrome P-450 CYP11B2, Receptor, Aldosterone, Functional implication, Articles, Cell Biology, Rats, Kidney Tubules, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, chemistry, Adrenal Medulla, Duct (anatomy) |
| الوصف: | (Pro)renin receptor (PRR) is predominantly expressed in the collecting duct (CD) with unclear functional implication. It is not known whether CD PRR is regulated by high potassium (HK). Here, we aimed to investigate the effect of HK on PRR expression and its role in regulation of aldosterone synthesis and release in the CD. In primary rat inner medullary CD cells, HK augmented PRR expression and soluble PPR (sPRR) release in a time- and dose-dependent manner, which was attenuated by PRR small interfering RNA (siRNA), eplerenone, and losartan. HK upregulated aldosterone release in parallel with an increase of CYP11B2 (cytochrome P-450, family 11, subfamily B, polypeptide 2) protein expression and upregulation of medium renin activity, both of which were attenuated by a PRR antagonist PRO20, PRR siRNA, eplerenone, and losartan. Similarly, prorenin upregulated aldosterone release and CYP11B2 expression, both of which were attenuated by PRR siRNA. Interestingly, a recombinant sPRR (sPRR-His) also stimulated aldosterone release and CYP11B2 expression. Taken together, we conclude that HK enhances a local renin-angiotensin-aldosterone system (RAAS), leading to increased PRR expression, which in turn amplifies the response of the RAAS, ultimately contributing to heightened aldosterone release. |
| تدمد: | 1522-1563 0363-6143 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c5c7bf43b6bd19dd1cc169185291137 https://doi.org/10.1152/ajpcell.00128.2016 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6c5c7bf43b6bd19dd1cc169185291137 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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