Dynamic evolution of emphysema and airway remodeling in two mouse models of COPD
| العنوان: | Dynamic evolution of emphysema and airway remodeling in two mouse models of COPD |
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| المؤلفون: | Tingting Di, Congli Fu, Yue Yang, Tao Bian, Zixiao Zhang, Jiaxin Liu, Yan Wu |
| المصدر: | BMC Pulmonary Medicine BMC Pulmonary Medicine, Vol 21, Iss 1, Pp 1-10 (2021) |
| بيانات النشر: | BioMed Central, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Lipopolysaccharides, Pathology, medicine.medical_specialty, Lipopolysaccharide, H&E stain, Inflammation, Pulmonary function testing, Cigarette Smoking, 03 medical and health sciences, chemistry.chemical_compound, Diseases of the respiratory system, Mice, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, medicine, Animals, 030304 developmental biology, Emphysema, 0303 health sciences, COPD, medicine.diagnostic_test, RC705-779, business.industry, Research, Chronic obstructive pulmonary disease, Cigarette smoke, Airway remodeling, respiratory system, medicine.disease, Staining, respiratory tract diseases, Mice, Inbred C57BL, Disease Models, Animal, Bronchoalveolar lavage, 030228 respiratory system, chemistry, Pulmonary Emphysema, medicine.symptom, Airway, business |
| الوصف: | Background Establishment of a mouse model is important for investigating the mechanism of chronic obstructive pulmonary disease (COPD). In this study, we observed and compared the evolution of the pathology in two mouse models of COPD induced by cigarette smoke (CS) exposure alone or in combination with lipopolysaccharide (LPS). Methods One hundred eight wild-type C57BL/6 mice were equally divided into three groups: the (1) control group, (2) CS-exposed group (CS group), and (3) CS + LPS-exposed group (CS + LPS group). The body weight of the mice was recorded, and noninvasive lung function tests were performed monthly. Inflammation was evaluated by counting the number of inflammatory cells in bronchoalveolar lavage fluid and measuring the expression of the IL-6 mRNA in mouse lung tissue. Changes in pathology were assessed by performing hematoxylin and eosin and Masson staining of lung tissue sections. Results The two treatments induced emphysema and airway remodeling and decreased lung function. Emphysema was induced after 1 month of exposure to CS or CS + LPS, while airway remodeling was induced after 2 months of exposure to CS + LPS and 3 months of exposure to CS. Moreover, the mice in the CS + LPS group exhibited more severe inflammation and airway remodeling than the mice in the CS group, but the two treatments induced similar levels of emphysema. Conclusion Compared with the single CS exposure method, the CS + LPS exposure method is a more suitable model of COPD in airway remodeling research. Conversely, the CS exposure method is a more suitable model of COPD for emphysema research due to its simple operation. |
| اللغة: | English |
| تدمد: | 1471-2466 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c938caf1e010bba524108acf570d07d http://europepmc.org/articles/PMC8073949 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6c938caf1e010bba524108acf570d07d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712466 |
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