Depression of Neuronal Protein Synthesis Initiation by Protein Tyrosine Kinase Inhibitors
| العنوان: | Depression of Neuronal Protein Synthesis Initiation by Protein Tyrosine Kinase Inhibitors |
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| المؤلفون: | Bing Ren Hu, Tadeusz Wieloch, Yi Bing Ou Yang |
| المصدر: | Journal of Neurochemistry. 61:1789-1794 |
| بيانات النشر: | Wiley, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | RNA, Transfer, Met, Cell Survival, Eukaryotic Initiation Factor-2, Nerve Tissue Proteins, Protein tyrosine phosphatase, Mitogen-activated protein kinase kinase, Biochemistry, Receptor tyrosine kinase, MAP2K7, Cellular and Molecular Neuroscience, Phenols, Leucine, Animals, ASK1, Carbon Radioisotopes, RNA, Messenger, Peptide Chain Initiation, Translational, Cells, Cultured, Protein Kinase C, Neurons, Analysis of Variance, MAP kinase kinase kinase, biology, Protein-Tyrosine Kinases, Embryo, Mammalian, Genistein, Isoflavones, Protein kinase R, Rats, Cinnamates, Protein Biosynthesis, biology.protein, Guanosine Triphosphate, Proto-oncogene tyrosine-protein kinase Src |
| الوصف: | Growth factors stimulate cellular protein synthesis, but the intracellular signaling mechanisms that regulate initiation of mRNA translation in neurons have not been clarified. A rate-limiting step in the initiation of protein synthesis is the formation of the ternary complex among GTP, eukaryotic initiation factor 2 (eIF-2), and the initiator tRNA. Here we report that genistein, a specific tyrosine kinase inhibitor, decreases tyrosine kinase activity and the content of phosphotyrosine proteins in cultured primary cortical neurons. Genistein inhibits protein synthesis by > 80% in a dose-dependent manner (10-80 micrograms/ml) and concurrently decreases ternary complex formation by 60%. At the doses investigated, genistein depresses tyrosine kinase activity and concomitantly stimulates PKC activity. We propose that a protein tyrosine kinase participates in the initiation of protein synthesis in neurons, by affecting the activity of eIF-2 directly or through a protein kinase cascade. |
| تدمد: | 1471-4159 0022-3042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cd24d1a4c3894c73a0d4de700e8373a https://doi.org/10.1111/j.1471-4159.1993.tb09817.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6cd24d1a4c3894c73a0d4de700e8373a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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