Decoding the Mechanism of Specific RNA Targeting by Ribosomal Methyltransferases
| العنوان: | Decoding the Mechanism of Specific RNA Targeting by Ribosomal Methyltransferases |
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| المؤلفون: | Juhi Singh, Rahul Raina, Kutti R. Vinothkumar, Ruchi Anand |
| المصدر: | ACS Chemical Biology. 17:829-839 |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | RNA, Ribosomal, RNA, Ribosomal, 16S, Cryoelectron Microscopy, Escherichia coli, RNA, Molecular Medicine, Methyltransferases, General Medicine, Ribosomes, Biochemistry |
| الوصف: | Methylation of specific nucleotides is integral for ribosomal biogenesis and also serves as a common mechanism to confer antibiotic resistance by pathogenic bacteria. Here, by determining the high-resolution structure of the 30S-KsgA complex by cryo-electron microscopy, a state was captured, where KsgA juxtaposes between helices h44 and h45 of the 30S ribosome, separating them, thereby enabling remodeling of the surrounded rRNA and allowing the cognate site to enter the methylation pocket. With the structure as a guide, several mutant versions of the ribosomes, where interacting bases in the catalytic helix h45 and surrounding helices h44, h24, and h27, were mutated and evaluated for their methylation efficiency revealing factors that direct the enzyme to its cognate site with high fidelity. The biochemical studies show that the three-dimensional environment of the ribosome enables the interaction of select loop regions in KsgA with the ribosome helices paramount to maintain selectivity. |
| تدمد: | 1554-8937 1554-8929 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d15ff068afd403f9e975cd8705210df https://doi.org/10.1021/acschembio.1c00732 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6d15ff068afd403f9e975cd8705210df |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15548937 15548929 |
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