First detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the Czech Republic
العنوان: | First detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the Czech Republic |
---|---|
المؤلفون: | Tomas Vesely, Stanislava Reschová, Jana Hulova, Monika Vicenova, Dagmar Pokorova |
المصدر: | Diseases of Aquatic Organisms. 95:87-95 |
بيانات النشر: | Inter-Research Science Center, 2011. |
سنة النشر: | 2011 |
مصطلحات موضوعية: | Barbel, Base Sequence, biology, Fishes, Zoology, Aquaculture, Vesiculovirus, Aquatic Science, biology.organism_classification, Barbus barbus, Cyprinus, Fishery, Fish Diseases, Common carp, Sturgeon, Rhabdoviridae Infections, Cyprinidae, Animals, Rhabdoviridae, Carp, computer, Phylogeny, Ecology, Evolution, Behavior and Systematics, Pike, computer.programming_language |
الوصف: | Rapid antigen detection enzyme-linked immunosorbent assay (ELISA) testing of cell cultures with organ homogenate from fish, collected from farms with a predominance of common carp or in natural aquaculture in the Czech Republic between 1995 and 2008, identified piscine vesiculovirus in 27 of 178 samples. Using reverse transcription semi-nested PCR, targeting a 550 nucleotide region of the glycoprotein (G) gene, piscine vesiculovirus was confirmed in 23 of the 27 organ samples diagnosed by ELISA as infected. PCR products were amplified and sequenced from 18 isolates from common carp Cyprinus carpio (family Cyprinidae), 2 isolates from northern pike Esox lucius (family Esocidae), and 1 isolate each from Siberian sturgeon Acipenser baerii (family Acipenseridae), common barbel Barbus barbus (family Cyprinidae), and koi carp Cyprinus carpio koi (family Cyprinidae). The sequences (based on 401 nucleotides) clustered into 2 genogroups. The majority of isolates (n = 22), including those from sturgeon and pike, grouped with the spring viraemia of carp virus (SVCV) Genogroup I and Subgroup Id. The 22 isolates could be further subdivided into 2 groups: Id1 (n = 20) and Id2 (n = 2). A marker (a non-conservative nucleotide substitution) for the Id1 SVCV group was identified. It was specifically found in all sequences of Id1 isolates when testing SVCV originating from different countries. The remaining isolate from barbel, was classified in the pike fry-like rhabdovirus Genogroup IV. This is the first confirmation of natural SVCV infection in sturgeon and pike, and pike fry-like rhabdovirus infection in barbel. In the case of the pike fry-like rhabdovirus, this is also its first identification in the Czech Republic. According to the presence/absence of evident clinical signs of rhabdoviral disease in the 3 infected hosts, only the sturgeon seemed to be susceptible to the monitored rhabdovirus. |
تدمد: | 1616-1580 0177-5103 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d1a48edd5131fe8b669f1d8322400c1 https://doi.org/10.3354/dao02340 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....6d1a48edd5131fe8b669f1d8322400c1 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 16161580 01775103 |
---|