Withdrawal: Salvianolic Acid B Protects SH-SY5Y Neuroblastoma Cells from 1-Methyl-4-phenylpyridinium-Induced Apoptosis
| العنوان: | Withdrawal: Salvianolic Acid B Protects SH-SY5Y Neuroblastoma Cells from 1-Methyl-4-phenylpyridinium-Induced Apoptosis |
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| المؤلفون: | Guang Zeng, Qing-hua Liang, Tao Tang, Yuan Lin, Wan-hui You, Qi-Dong Yang, Xing-Qun Li, Han-jun Wu, Xi Huang, Jiekun Luo |
| المصدر: | Biological and Pharmaceutical Bulletin. 33:1337-1342 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | 1-Methyl-4-phenylpyridinium, SH-SY5Y, Cell Survival, Cell Culture Techniques, Pharmaceutical Science, Apoptosis, Pharmacology, Biology, Mitochondrion, medicine.disease_cause, Neuroprotection, Antiparkinson Agents, Neuroblastoma, Cell Line, Tumor, parasitic diseases, medicine, Humans, Viability assay, Benzofurans, bcl-2-Associated X Protein, Membrane Potential, Mitochondrial, chemistry.chemical_classification, Reactive oxygen species, Molecular Structure, Caspase 3, Reverse Transcriptase Polymerase Chain Reaction, Cytochrome c, Parkinson Disease, General Medicine, Molecular biology, chemistry, Proto-Oncogene Proteins c-bcl-6, biology.protein, Biological Assay, Reactive Oxygen Species, Oxidative stress |
| الوصف: | Parkinson's disease (PD) is associated with mitochondrial dysfunction, oxidative stress, and activation of the apoptotic cascade. In the study, we investigated the effects of salvianolic acid B (Sal B) on 1-methyl-4-phenylpyridinium (MPP(+))-treated SH-SY5Y cells, a classic in vitro model for PD. We found Sal B inhibited the loss of cell viability by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The underlying mechanisms of Sal B action were further studied. Treatment of SH-SY5Y cells with MPP(+) caused a loss of cell viability and mitochondrial membrane potential, condensation of nuclei, elevation in the level of reactive oxygen species (which was associated with cytochrome c release), an increase in the Bax/Bcl-2 mRNA ratio, and activation of caspase-3. Sal B ameliorated the MPP(+)-altered phenotypes. These results indicate that the Sal B protected SH-SY5Y cells against MPP(+)-induced apoptosis by relieving oxidative stress and modulating the apoptotic process. Our findings suggest that salvianolic acid B may be a promising agent to prevent PD. |
| تدمد: | 1347-5215 0918-6158 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d2cec9e7c29b116387dcd0a2be2aa03 https://doi.org/10.1248/bpb.33.1337 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6d2cec9e7c29b116387dcd0a2be2aa03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
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