Long Non-Coding RNA NEAT1 Regulates Pyroptosis in Diabetic Nephropathy via Mediating the miR-34c/NLRP3 Axis
| العنوان: | Long Non-Coding RNA NEAT1 Regulates Pyroptosis in Diabetic Nephropathy via Mediating the miR-34c/NLRP3 Axis |
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| المؤلفون: | Chong Huang, Hong-Wei Huang, Wen-Wei Xu, Li-Li Hu, Jin-Feng Zhan |
| المصدر: | Kidney & Blood Pressure Research, Vol 45, Iss 4, Pp 589-602 (2020) |
| بيانات النشر: | Karger Publishers, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, lcsh:Diseases of the circulatory (Cardiovascular) system, Cell, 030232 urology & nephrology, Inflammation, Biology, lcsh:RC870-923, Rats, Sprague-Dawley, Pathogenesis, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Western blot, NLR Family, Pyrin Domain-Containing 3 Protein, medicine, lcsh:Dermatology, Animals, Diabetic Nephropathies, Viability assay, nlrp3, medicine.diagnostic_test, mir-34c, pyroptosis, Pyroptosis, General Medicine, Transfection, lcsh:RL1-803, lcsh:Diseases of the genitourinary system. Urology, Rats, Cell biology, MicroRNAs, medicine.anatomical_structure, Gene Expression Regulation, Nephrology, inflammation, lcsh:RC666-701, neat1, RNA, Long Noncoding, medicine.symptom, Cardiology and Cardiovascular Medicine |
| الوصف: | Introduction: Diabetic nephropathy (DN) is a serious complication of diabetes mellitus and is considered to be a sterile inflammatory disease. Increasing evidence suggest that pyroptosis and subsequent inflammatory response play a key role in the pathogenesis of DN. However, the underlying cellular and molecular mechanisms responsible for pyroptosis in DN are largely unknown. Methods: The rat models of DN were successfully established by single 65 mg/kg streptozotocin treatment. Glomerular mesangial cells were exposed to 30 mmol/L high glucose media for 48 h to mimic the DN environment in vitro. Gene and protein expressions were determined by quantitative real-time PCR and Western blot. Cell viability and pyroptosis were measured by MTT assay and flow cytometry analysis, respectively. The relationship between lncRNA NEAT1, miR-34c, and Nod-like receptor protein-3 (NLRP3) was confirmed by luciferase reporter assay. Results: We found that upregulation of NEAT1 was associated with the increase of pyroptosis in DN models. miR-34c, as a target gene of NEAT1, mediated the effect of NEAT1 on pyroptosis in DN by regulating the expression of NLRP3 as well as the expressions of caspase-1 and interleukin-1β. Either miR-34c inhibition or NLRP3 overexpression could reverse the accentuation of pyroptosis and inflammation by sh-NEAT1 transfection in the in vitro model of DN. Conclusions: Our findings suggested NEAT1 and its target gene miR-34c regulated cell pyroptosis via mediating NLRP3 in DN, providing new insights into understanding the molecular mechanisms of pyroptosis in the pathogenesis of DN. |
| اللغة: | English |
| تدمد: | 1423-0143 1420-4096 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d35dce70bd276a43ff49710445fe2fb https://www.karger.com/Article/FullText/508372 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6d35dce70bd276a43ff49710445fe2fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230143 14204096 |
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