An in vitro/in vovo screening assay as a sensitive tool to assess endocrine disruptive activity in surface water
| العنوان: | An in vitro/in vovo screening assay as a sensitive tool to assess endocrine disruptive activity in surface water |
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| المؤلفون: | Bart van der Burg, Selinda de Vries-Buitenweg, Sander C. van der Linden, L.M. Puijker, Juliette Legler, Raoul V. Kuiper, Albertinka J. Murk, Rinus Bogers, Beppy van de Waart, Ine Geuijen |
| المساهمون: | Philosophy, Chemistry and Biology, Institute for Environmental Studies, Advances in Veterinary Medicine, Dep Pathobiologie |
| المصدر: | Environment International, 33(3), 292-301 Environment International 33 (2007) 3 Environment International, 33(3), 292-301. Elsevier Limited Environment International, Vol 33, Iss 3, Pp 292-301 (2007) Borgers, R, De Vries-Buitenweg, S, Geuijen, I, De Roode, D, Kuiper, R, Van der Linden, S, Puiker, L, Murk, A J, Van der Burg, B & Legler, J 2007, ' An in vitro/in vivo screening assay as a sensitive tool to assess endocrine disruptive activity in surface water ', Environment International, vol. 33, no. 3, pp. 292-301 . https://doi.org/10.1016/j.envint.2006.10.002 |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, fathead minnow, Endocrine Disruptors, Toxicology, Vitellogenins, Blood plasma, Bioassay, cyprinodon-variegatus, lcsh:Environmental sciences, General Environmental Science, media_common, Netherlands, lcsh:GE1-350, biology, Minnow, Wageningen Marine Research, Liver, Receptors, Estrogen, medaka oryzias-latipes, secondary sex characteristics, Biological Assay, Reproduction, SDG 6 - Clean Water and Sanitation, media_common.quotation_subject, Cyprinidae, minnow pimephales-promelas, Andrology, Vitellogenin, synthetic estrogen, Rivers, In vivo, biology.animal, Cell Line, Tumor, Animals, Humans, RNA, Messenger, vitellogenin messenger-rna, Gonads, estrogenic activity, treated sewage effluent, Toxicologie, WIMEK, biology.organism_classification, Sheepshead minnow, rainbow-trout, WOT Natuur & Milieu, biology.protein, Water Pollutants, Chemical |
| الوصف: | Adult male fathead minnow were exposed for 14 or 28-days under flow-through conditions to undiluted filtered water samples from the rivers Meuse and Rhine in the Netherlands. The experiment included two vessels per treatment each containing 10 fish and samples of five fish were taken after 14 and 28 days. Additional groups were exposed to 17α-ethinylestradiol (EE2) as a reference and untreated drinking water as a negative control. Major endpoints examined included induction of vitellogenin (VTG) synthesis, VTG mRNA activity, hepato- and gonadosomatic indices (HSI and GSI) and gonadal histology. No significant difference was recorded in body weight or mean GSI values between the various treatments. Only exposure to Meuse water resulted in significantly higher HSI means after 14 days. Histological examination showed no apparent effects on gonadal tissue except for eosinophilic blood plasma in fish exposed to Meuse water or EE2. After 14 and 28 days, elevated VTG and VTG mRNA levels were measured in most livers of the fish exposed to Meuse water, but not in the fish exposed to Rhine water. This was confirmed by measuring estrogenic responses in the in vitro ER CALUX® assay. Induction of VTG synthesis proved to be the most sensitive endpoint in the Non Spawning Male Fish Assay for in vivo detection of bio-available estrogenic activity supplementary to a sensitive in vitro assay. The other endpoints examined varied too much and required a higher number of fish or replicates to achieve sufficient power for statistical testing making them less animal friendly. Keywords: Estrogenic activity, Non spawning male fish assay, Fathead minnow, Vitellogenin, River waters, Drinking water |
| وصف الملف: | application/octet-stream; application/pdf; image/pdf |
| اللغة: | English |
| تدمد: | 0160-4120 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d5a0de6e58d9be6c719f3761d7f4fc3 https://doi.org/10.1016/j.envint.2006.10.002 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6d5a0de6e58d9be6c719f3761d7f4fc3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01604120 |
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