Characteristics of a Novel Monoclonal Antibody Against Interleukin-14α
| العنوان: | Characteristics of a Novel Monoclonal Antibody Against Interleukin-14α |
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| المؤلفون: | Changhua Zhou, Xiao-dong Peng, Chongjie Zhang, Zhijuan Luo, Dapeng Wei |
| المصدر: | Hybridoma. 28:235-239 |
| بيانات النشر: | Mary Ann Liebert Inc, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | medicine.drug_class, medicine.medical_treatment, Immunology, Vesicular Transport Proteins, Mice, Transgenic, Monoclonal antibody, Autoimmune Diseases, Mice, Immune system, Antigen, Western blot, medicine, Animals, Humans, Immunoprecipitation, Immunology and Allergy, Autoimmune disease, Mice, Inbred BALB C, biology, medicine.diagnostic_test, Interleukins, Antibodies, Monoclonal, Interleukin, Exons, medicine.disease, Molecular biology, Kinetics, Cytokine, Immune System, Immunoglobulin G, biology.protein, Female, Antibody, Multiple Myeloma |
| الوصف: | Interleukin-14alpha is cytokine encoded by the plus strand of il-14 gene using exons 3-10. It has been demonstrated in an IL-14-transgenic mice model that IL-14alpha could enhance an immune response to both TD and TI antigens and induce a phenotype that is very similar to SLE and Sjögren's syndrome, indicating that IL-14alpha may play an important role in autoimmune disease. To further study the role of IL-14alpha in human immune diseases, there is an urgent need to generate specific anti-human IL-14alpha monoclonal antibodies. In this study, we immunized BALB/c mice with synthesized human IL-14alpha-C polypeptide and obtained a novel monoclonal antibody, the anti-IL-14alpha-C antibody. Analysis by enzyme-linked immunosorbent assay (ELISA), spot-ELISA, Western blot, and immuoprecipitation revealed that the MAb were specifically against recombinant human IL-14alpha protein, and its subtype and affinity constant (K(aff)) were IgG2a/kappa and 1.007 x 10(8) M(-1), respectively. This antibody might facilitate studies for pathophysiology of autoimmune disease. |
| تدمد: | 1557-8348 1554-0014 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d806b46f635e2a1beb3a4768e657809 https://doi.org/10.1089/hyb.2009.0007 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6d806b46f635e2a1beb3a4768e657809 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578348 15540014 |
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