Granulosa cells in three‐dimensional culture: A follicle‐like structure for domestic cat vitrified oocytes
| العنوان: | Granulosa cells in three‐dimensional culture: A follicle‐like structure for domestic cat vitrified oocytes |
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| المؤلفون: | Gaia Cecilia Luvoni, Maria Giorgia Morselli, Martina Colombo, Maricy Apparicio |
| المساهمون: | Università degli Studi di Milano, Universidade de Franca (UNIFRAN), Universidade Estadual Paulista (Unesp) |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Alginates, cryopreservation, Andrology, 03 medical and health sciences, Follicle, 0302 clinical medicine, Endocrinology, Meiosis, Animals, Secretion, feline, Cells, Cultured, Progesterone, Cryopreservation, barium alginate, Granulosa Cells, 030219 obstetrics & reproductive medicine, Estradiol, biology, Felis, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Progesterone secretion, in vitro maturation, biology.organism_classification, co-culture, Vitrification, 040201 dairy & animal science, Coculture Techniques, In vitro, In Vitro Oocyte Maturation Techniques, In vitro maturation, Cats, Female, Animal Science and Zoology, 3D, Biotechnology, Hormone |
| الوصف: | Made available in DSpace on 2020-12-12T01:12:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01 Follicle-like structures are three-dimensional matrices joint with living cells that allow the in vitro development of female gametes in more physiological conditions. They have been shown to be beneficial to fresh oocytes in different species, and in this study, domestic cat (Felis silvestris catus) granulosa cells were used to create a functional follicle-like structure aimed at supporting the in vitro maturation of conspecific vitrified oocytes, key players of fertility preservation programmes that usually struggle to acquire their full developmental competence after warming. Cat granulosa cells were cultured for up to 6 days in three-dimensional barium alginate microcapsules (i.e. follicle-like structures) or in two-dimensional monolayers, and their steroidogenic ability (estradiol and progesterone secretion) was assessed to confirm their functionality. The same systems were used (on day 2 or 6 of granulosa cells culture) for the in vitro maturation (IVM) of Cryotop® vitrified immature cat oocytes and compared with microdrops of IVM medium without cells (control). Granulosa cells were able to maintain their functionality in vitro in both the conditions, even if with a different extent of hormonal secretion along culture (p =.02). Vitrified oocytes resumed meiosis at higher rates when cultured with 2 days old granulosa cells (p =.03), but full maturation rates slightly raised when granulosa cells were cultured longer, albeit without differences with the control group. This study paved the road for the creation of enriched culture systems in the domestic cat, but innovations are strongly needed for vitrified oocytes that deserve better chances to develop in vitro. Dipartimento di Scienze Veterinarie per la Salute la Produzione Animale e la Sicurezza Alimentare “Carlo Cantoni” Università degli Studi di Milano Programa de Mestrado em Ciência Animal Universidade de Franca (UNIFRAN) Departamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista Julio de Mesquita Filho (UNESP) Departamento de Medicina Veterinária Preventiva e Reprodução Animal Universidade Estadual Paulista Julio de Mesquita Filho (UNESP) |
| تدمد: | 1439-0531 0936-6768 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d890f26e05a5fb78342904696d17d84 https://doi.org/10.1111/rda.13597 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6d890f26e05a5fb78342904696d17d84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390531 09366768 |
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