D5dopamine receptor regulation of phospholipase D
| العنوان: | D5dopamine receptor regulation of phospholipase D |
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| المؤلفون: | David R. Sibley, Zhiwei Yang, Ren Kui Bai, Laureano D. Asico, John E. Jones, Robin A. Felder, Pedro A. Jose, Peiying Yu, Zheng Wang |
| المصدر: | American Journal of Physiology-Heart and Circulatory Physiology. 288:H55-H61 |
| بيانات النشر: | American Physiological Society, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Gene isoform, medicine.medical_specialty, Vascular smooth muscle, Physiology, Blood Pressure, CHO Cells, Biology, Kidney, medicine.disease_cause, Cell Line, Mice, Cricetulus, Cricetinae, Physiology (medical), Internal medicine, Phospholipase D, medicine, Animals, Humans, Receptors, Dopamine D5, Tissue Distribution, RNA, Messenger, Receptor, Mice, Knockout, Receptors, Dopamine D1, biology.organism_classification, Endocrinology, Cell culture, Dopamine receptor, Cardiology and Cardiovascular Medicine, Oxidative stress |
| الوصف: | D1-like receptors have been reported to decrease oxidative stress in vascular smooth muscle cells by decreasing phospholipase D (PLD) activity. However, the PLD isoform regulated by D1-like receptors (D1or D5) and whether abnormal regulation of PLD by D1-like receptors plays a role in the pathogenesis of hypertension are unknown. The hypothesis that the D5receptor is the D1-like receptor that inhibits PLD activity and serves to regulate blood pressure was tested using D5receptor mutant mice (D5−/−). We found that in the mouse kidney, PLD2, like the D5receptor, is mainly expressed in renal brush-border membranes, whereas PLD1 is mainly expressed in renal vessels with faint staining in brush-border membranes and collecting ducts. Total renal PLD activity is increased in D5−/−mice relative to congenic D5wild-type (D5+/+) mice. PLD2, but not PLD1, expression is greater in D5−/−than in D5+/+mice. The D5receptor agonist fenoldopam decreases PLD2, but not PLD1, expression and activity in human embryonic kidney-293 cells heterologously expressing the human D5receptor, effects that are blocked by the D5receptor antagonist SCH-23390. These studies show that the D5receptor regulates PLD2 activity and expression. The hypertension in the D5−/−mice is associated with increased PLD expression and activity. Impaired D5receptor regulation of PLD2 may play a role in the pathogenesis of hypertension. |
| تدمد: | 1522-1539 0363-6135 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6df1c7e1294e9eb9183c9a0d6c25821e https://doi.org/10.1152/ajpheart.00627.2004 |
| رقم الأكسشن: | edsair.doi.dedup.....6df1c7e1294e9eb9183c9a0d6c25821e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221539 03636135 |
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