Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type FgTri1 Gene
| العنوان: | Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type FgTri1 Gene |
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| المؤلفون: | Shuichi Ohsato, Kazuyuki Maeda, Naoko Takahashi-Ando, Makoto Kimura, Yuichi Nakajima, Yoshiaki Koizumi |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 11428, p 11428 (2021) International Journal of Molecular Sciences |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Fusarium, Fusarium mycotoxin, QH301-705.5, Trichothecene, Catalysis, Microbiology, Inorganic Chemistry, Hydroxylation, Fungal Proteins, chemistry.chemical_compound, cytochrome P450 monooxygenase gene, Cytochrome P-450 Enzyme System, evolution, Physical and Theoretical Chemistry, Biology (General), Axenic, Molecular Biology, QD1-999, Spectroscopy, Chemotype, biology, Strain (chemistry), Organisms, Genetically Modified, Chemistry, Axenic Culture, Communication, Organic Chemistry, Cytochrome P450, General Medicine, Monooxygenase, biology.organism_classification, trichothecene chemotype, Computer Science Applications, biology.protein, NX-type trichothecenes, Trichothecenes |
| الوصف: | Fusarium graminearum species complex produces type B trichothecenes oxygenated at C-7. In axenic liquid culture, F. graminearum mainly accumulates one of the three types of trichothecenes, namely 3-acetyldeoxyinvalenol, 15-acetyldeoxyinvalenol, or mixtures of 4,15-diacetylnivalenol/4-acetylnivalenol, depending on each strain’s genetic background. The acetyl groups of these trichothecenes are slowly deacetylated to give deoxynivalenol (DON) or nivalenol (NIV) on solid medium culture. Due to the evolution of F. graminearum FgTri1, encoding a cytochrome P450 monooxygenase responsible for hydroxylation at both C-7 and C-8, new derivatives of DON, designated as NX-type trichothecenes, have recently emerged. To assess the risks of emergence of new NX-type trichothecenes, we examined the effects of replacing FgTri1 in the three chemotypes with FgTri1_NX chemotype, which encodes a cytochrome P450 monooxygenase that can only hydroxylate C-7 of trichothecenes. Similar to the transgenic DON chemotypes, the transgenic NIV chemotype strain accumulated NX-type 4-deoxytrichothecenes in axenic liquid culture. C-4 oxygenated trichothecenes were marginal, despite the presence of a functional FgTri13 encoding a C-4 hydroxylase. At present, outcrossing of the currently occurring NX chemotype with NIV chemotype strains of F. graminearum in the natural environment likely will not yield a new strain that produces a C-4 oxygenated NX-type trichothecene. |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e6fae1f1d873c3f8f24dbcba15c0ed0 https://www.mdpi.com/1422-0067/22/21/11428 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6e6fae1f1d873c3f8f24dbcba15c0ed0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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