Methods for isolation of messenger RNA from biological samples
| العنوان: | Methods for isolation of messenger RNA from biological samples |
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| المؤلفون: | Bi-Feng Yuan, Wan-Bing Tao, Yu-Qi Feng, Ming-Yu Cheng |
| المصدر: | Analytical Methods. 13:289-298 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Regulation of gene expression, 0303 health sciences, Messenger RNA, General Chemical Engineering, General Engineering, Eukaryota, Gene Expression, RNA, Central dogma of molecular biology, Computational biology, Biology, 010402 general chemistry, Isolation (microbiology), 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, 03 medical and health sciences, Eukaryotic Cells, Research studies, RNA, Messenger, Molecular Biology, 030304 developmental biology |
| الوصف: | RNA molecules contain many chemical modifications that can regulate a variety of biological processes. Messenger RNA (mRNA) molecules are critical components in the central dogma of molecular biology. The discovery of reversible chemical modifications in eukaryotic mRNA brings forward a new research field in RNA modification-mediated regulation of gene expression. The modifications in mRNA generally exist in low abundance. The use of highly pure mRNA is critical for the confident identification of new modifications as well as for the accurate quantification of existing modifications in mRNA. In addition, isolation of highly pure mRNA is the first step in many biological research studies. Therefore, the methods for isolating highly pure mRNA are important for mRNA-based downstream studies. A variety of methods for isolating mRNA have been developed in the past few decades and new methods continuously emerge. This review focuses on the methodologies and protocols for isolating mRNA populations. In addition, we discuss the advantages and limitations of these methods. We hope this paper will provide a general view of mRNA isolation strategies and facilitate studies that involve mRNA modifications and functions. |
| تدمد: | 1759-9679 1759-9660 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e9b34e71b0c81a8c7ec7c9655472f11 https://doi.org/10.1039/d0ay01912g |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6e9b34e71b0c81a8c7ec7c9655472f11 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17599679 17599660 |
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