Quantitative proteomic profiling of extracellular matrix and site-specific collagen post-translational modifications in an in vitro model of lung fibrosis
| العنوان: | Quantitative proteomic profiling of extracellular matrix and site-specific collagen post-translational modifications in an in vitro model of lung fibrosis |
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| المؤلفون: | Juliane Merl-Pham, Larissa Knüppel, Oliver Eickelberg, Claudia A. Staab-Weijnitz, Mark Athanason, Stefan Engelhardt, Roberto M. Vanacore, Trayambak Basak, Deepak Ramanujam, Stefanie M. Hauck, Jürgen Behr |
| المصدر: | Matrix biology plus 1, DOI: 10.1016/j.mbplus.2019.04.002:100005 (2019) Matrix Biology Plus, Vol 1, Iss, Pp-(2019) Matrix Biology Plus |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | PTM, post-translational modification, COL1A1, collagen-I alpha 1 chain, Proteomics, Biochemistry, Extracellular matrix, Idiopathic pulmonary fibrosis, chemistry.chemical_compound, Pulmonary fibrosis, HyP, hydroxyproline, lcsh:QH301-705.5, TGF-β, transforming growth factor β, PLOD (LH), procollagen-lysine,2-oxoglutarate 5-dioxygenases (lysyl hydroxylases), AGC, automatic gain control, Lysyl glycosylation, Transforming growth factor-β, Yaa, Yaa position in the Gly-Xaa-Yaa repeat in triple-helical collagen, Cell biology, ECM, extracellular matrix, LH, lysyl hydroxylase, PAI1, plasminogen activator inhibitor 1, Collagen, TGM2, transglutaminase 1, ANXA11, annexin A11, Type I collagen, Histology, Glycosylation, DCN, decorin, Biophysics, VCAN, versican, Article, P4H, prolyl-4-hydroxylase, HyK, hydroxylysine, α-SMA, α-smooth muscle actin, SEMA7A, semaphorin 7a, LTBP2, latent-transforming growth factor β -binding protein 2, Genetics, medicine, 4-HyP, 4-hydroxyproline, IPF, idiopathic pulmonary fibrosis, Collagen post-translational modifications, Molecular Biology, G-HyK, galactosylhydroxylysine, LOX(L), lysyl oxidase(-like), PCA, principal component analysis, Prolyl hydroxylation, BGN, biglycan, GG-HyK, glucosylgalactosylhydroxylysine, Proteomic Profiling, Xaa, Xaa position in the Gly-Xaa-Yaa repeat in triple-helical collagen, Cell Biology, P3H, prolyl-3-hydroxylase, medicine.disease, FN1, fibronectin 1, chemistry, 3-HyP, 3-hydroxyproline, lcsh:Biology (General), ILD, interstitial lung disease, Lysyl hydroxylation, Transforming growth factor |
| الوصف: | Lung fibrosis is characterized by excessive deposition of extracellular matrix (ECM), in particular collagens, by fibroblasts in the interstitium. Transforming growth factor-β1 (TGF-β1) alters the expression of many extracellular matrix (ECM) components produced by fibroblasts, but such changes in ECM composition as well as modulation of collagen post-translational modification (PTM) levels have not been comprehensively investigated. Here, we performed mass spectrometry (MS)-based proteomics analyses to assess changes in the ECM deposited by cultured lung fibroblasts from idiopathic pulmonary fibrosis (IPF) patients upon stimulation with transforming growth factor β1 (TGF-β1). In addition to the ECM changes commonly associated with lung fibrosis, MS-based label-free quantification revealed profound effects on enzymes involved in ECM crosslinking and turnover as well as multiple positive and negative feedback mechanisms of TGF-β1 signaling. Notably, the ECM changes observed in this in vitro model correlated significantly with ECM changes observed in patient samples. Because collagens are subject to multiple PTMs with major implications in disease, we implemented a new bioinformatic platform to analyze MS data that allows for the comprehensive mapping and site-specific quantitation of collagen PTMs in crude ECM preparations. These analyses yielded a comprehensive map of prolyl and lysyl hydroxylations as well as lysyl glycosylations for 15 collagen chains. In addition, site-specific PTM analysis revealed novel sites of prolyl-3-hydroxylation and lysyl glycosylation in type I collagen. Interestingly, the results show, for the first time, that TGF-β1 can modulate prolyl-3-hydroxylation and glycosylation in a site-specific manner. Taken together, this proof of concept study not only reveals unanticipated TGF-β1 mediated regulation of collagen PTMs and other ECM components but also lays the foundation for dissecting their key roles in health and disease. The proteomic data has been deposited to the ProteomeXchange Consortium via the MassIVE partner repository with the data set identifier MSV000082958. Highlights • Quantitative proteomics of TGF-β-induced changes in ECM composition and collagen PTM in pulmonary fibroblasts • TGF-β promotes crosslinking and turnover as well as complex feedback mechanisms that alter fibroblast ECM homeostasis. • A novel bioinformatic workflow for MS data analysis enabled global mapping and quantitation of known and novel collagen PTMs • Quantitative assessment of prolyl-3-hydroxylation site occupancy and lysine-O-glycosylation microheterogeneity • TGF-β1 modulates collagen PTMs in a site-specific manner that may favor collagen accumulation in lung fibrosis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ef78907859c6ce89234695b5deacfc4 https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=56657 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6ef78907859c6ce89234695b5deacfc4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |