Evidence that deoxyribonucleic acid photolyase from bakers' yeast is a flavoprotein
| العنوان: | Evidence that deoxyribonucleic acid photolyase from bakers' yeast is a flavoprotein |
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| المؤلفون: | H Werbin, C O Joe, N Iwatsuki |
| المصدر: | Biochemistry. 19:1172-1176 |
| بيانات النشر: | American Chemical Society (ACS), 1980. |
| سنة النشر: | 1980 |
| مصطلحات موضوعية: | Flavin adenine dinucleotide, Gel electrophoresis, Protein Denaturation, Flavoproteins, biology, Flavin Mononucleotide, Size-exclusion chromatography, Lyases, Flavoprotein, Saccharomyces cerevisiae, Flavin group, DNA photolyase, Biochemistry, chemistry.chemical_compound, Spectrometry, Fluorescence, chemistry, Spectrophotometry, Flavin-Adenine Dinucleotide, biology.protein, Denaturation (biochemistry), Photolyase, Deoxyribodipyrimidine Photo-Lyase |
| الوصف: | DNA photolyase purified from baker's yeast by affinity chromatography on UV-irradiated DNA noncovalently bound to cellulose and by chromatography on activated thiol-Sepharose 4B yields a single protein band having a molecular weight of 51 000 when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight, 53 000, determined by gel filtration was in good agreement. Upon denaturation of photolyase by heat or 8 M urea, flavin adenine dinucleotide (oxidized) was isolated from the mixture and identified by thin-layer chromatography and spectral analysis. In contrast to flavoproteins to which flavin adenine dinucleotide (oxidized) is bound which generally exhibit two absorbance maxima between 300 and 500 nm, photolyase has only one at 380 nm. These findings and the similar characteristics of the absorbance and emission spectra of native photolyase with those of flavoproteins in which the chromophore is considered to be the 4a,5-reduced flavin have led us to propose this configuration for the photolyase chromophore. The difference in properties of yeast photolyase compared to the one reported previously supports the idea that there are two photolyases in baker's yeast. |
| تدمد: | 1520-4995 0006-2960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6efca2b752a31be31f71b3b7fbffc42b https://doi.org/10.1021/bi00547a021 |
| رقم الأكسشن: | edsair.doi.dedup.....6efca2b752a31be31f71b3b7fbffc42b |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204995 00062960 |
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