Polymorphisms within the ARNT2 and CX3CR1 Genes Are Associated with the Risk of Developing Invasive Aspergillosis
| العنوان: | Polymorphisms within the ARNT2 and CX3CR1 Genes Are Associated with the Risk of Developing Invasive Aspergillosis |
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| المؤلفون: | Lupiañez, C B, Martínez-Bueno, M, Sánchez-Maldonado, J M, Badiola, J, Cunha, C, Springer, J, Lackner, M, Segura-Catena, J, Canet, L M, Alcazar-Fuoli, L, López-Nevot, M A, Fianchi, L, Aguado, J M, Pagano, L, López-Fernández, E, Alarcón-Riquelme, M, Potenza, L, Gonçalves, S M, Luppi, M, Moratalla, L, Solano, C, Sampedro, A, González-Sierra, P, Cuenca-Estrella, M, Lagrou, K, Maertens, J A, Lass-Flörl, C, Einsele, H, Vazquez, L, PCRAGA Study Group, Loeffler, J, Ríos-Tamayo, R, Carvalho, A, Jurado, M, Sainz, J |
| المصدر: | INFECTION AND IMMUNITY r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA instname |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, host immunity, Genotype, 030106 microbiology, Immunology, Population, CX3C Chemokine Receptor 1, Single-nucleotide polymorphism, ARNT2, CX3CR1, genetic susceptibility, invasive aspergillosis, Biology, Aspergillosis, Microbiology, Polymorphism, Single Nucleotide, Risk Assessment, 03 medical and health sciences, medicine, Genetic predisposition, Basic Helix-Loop-Helix Transcription Factors, Humans, Genetic Predisposition to Disease, Allele, education, Invasive Pulmonary Aspergillosis, education.field_of_study, Haplotype, Aryl Hydrocarbon Receptor Nuclear Translocator, PCRAGA Study Group, medicine.disease, Hematologic Diseases, 3. Good health, Settore MED/15 - MALATTIE DEL SANGUE, 030104 developmental biology, Infectious Diseases, Aspergillus, Case-Control Studies, Expression quantitative trait loci, Parasitology |
| الوصف: | Invasive aspergillosis (IA) is a life-threatening infection that affects an increasing number of patients undergoing chemotherapy or allo-transplantation, and recent studies have shown that genetic factors contribute to disease susceptibility. In this two-stage, population-based, case-control study, we evaluated whether 7 potentially functional single nucleotide polymorphisms (SNPs) within the ARNT2 and CX3CR1 genes influence the risk of IA in high-risk hematological patients. We genotyped selected SNPs in a cohort of 500 hematological patients (103 of those had been diagnosed with proven or probable IA), and we evaluated their association with the risk of developing IA. The association of the most interesting markers of IA risk was then validated in a replication population, including 474 subjects (94 IA and 380 non-IA patients). Functional experiments were also performed to confirm the biological relevance of the most interesting markers. The meta-analysis of both populations showed that carriers of the ARNT2rs1374213G, CX3CR1rs7631529A, and CX3CR1rs9823718G alleles (where the RefSeq identifier appears as a subscript) had a significantly increased risk of developing IA according to a log-additive model (P value from the meta-analysis [PMeta] = 9.8 · 10-5, PMeta = 1.5 · 10-4, and PMeta =7.9 · 10-5, respectively). Haplotype analysis also confirmed the association of the CX3CR1 haplotype with AG CGG with an increased risk of IA (P = 4.0 · 10-4). Mechanistically, we observed that monocyte-derived macrophages (MDM) from subjects carrying the ARNTR2rs1374213G allele or the GG genotype showed a significantly impaired fungicidal activity but that MDM from carriers of the ARNT2rs1374213G and CX3CR1rs9823718G or CX3CR1rs7631529A alleles had deregulated immune responses to Aspergillus conidia. These results, together with those from expression quantitative trait locus (eQTL) data browsers showing a strong correlation of the CX3CR1rs9823718G allele with lower levels of CX3CR1 mRNA in whole peripheral blood (P = 2.46 · 10-7) and primary monocytes (P = 4.31 · 10-7), highlight the role of the ARNT2 and CX3CR1 loci in modulating and predicting IA risk and provide new insights into the host immune mechanisms involved in IA development. ispartof: INFECTION AND IMMUNITY vol:88 issue:4 ispartof: location:United States status: published |
| وصف الملف: | Electronic-Print |
| اللغة: | English |
| تدمد: | 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f43290f6aa91b179dcb728a0944b95f https://hdl.handle.net/10668/14981 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6f43290f6aa91b179dcb728a0944b95f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00199567 |
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