5-aminolaevulinic acid photodynamic therapy in a transgenic mouse model of skin melanoma
| العنوان: | 5-aminolaevulinic acid photodynamic therapy in a transgenic mouse model of skin melanoma |
|---|---|
| المؤلفون: | Lasse R. Braathen, Masashi Kato, Joachim Weis, Claudio Vallan, V. von Felbert, Izumi Nakashima, F Córdoba, Jakob Weissenberger |
| المصدر: | Experimental Dermatology. 14:429-437 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Genetically modified mouse, Pathology, medicine.medical_specialty, Skin Neoplasms, Light, Cell Survival, medicine.medical_treatment, Melanoma, Experimental, Tetrazolium Salts, Mice, Transgenic, Photodynamic therapy, Dermatology, Biology, Biochemistry, Transgenic Model, Flow cytometry, Mice, In vivo, Cell Line, Tumor, medicine, Animals, Humans, Neoplasm Metastasis, Melanoma, neoplasms, Molecular Biology, Photosensitizing Agents, medicine.diagnostic_test, Aminolevulinic Acid, Neoplasms, Experimental, Trypan Blue, Flow Cytometry, medicine.disease, Fibrosis, In vitro, Disease Models, Animal, Thiazoles, Photochemotherapy, Cell culture, NIH 3T3 Cells |
| الوصف: | Photodynamic therapy (PDT) is widely used to treat preneoplastic skin lesions and non-melanoma skin tumours. Studies analyzing the effects of PDT on malignant melanoma have yielded conflicting results. On the one hand, melanoma cell lines in culture as well as cell lines transplanted into experimental animals were sensitive to PDT. On the other hand, spontaneous melanomas of human patients responded poorly to most PDT regimens tested so far. Here, we analyzed effects of 5-aminolaevulinic acid (5-ALA)-based PDT on melanoma cell lines and on experimental melanomas. To mimic the clinical situation as closely as possible, metallothionein-I/ret (MT-ret) mice, a transgenic model of skin melanoma development, were used. Optimal doses of 5-ALA as well as energy doses and power densities were determined in vitro using a cell line (Mel25) established by us from a melanoma of an MT-ret transgenic mouse as well as commercially available human and mouse melanoma cell lines. Treatment with light irradiation alone had no effect. In combination with 5-ALA, however, this illumination readily induced the death of all mouse and human melanoma cell lines examined. Still, 5-ALA PDT caused only minor focal regressive changes including haemorrhages and fibrosis of MT-ret melanomas in vivo and did not significantly delay tumour growth. These results show that, even though MT-ret melanoma cells are vulnerable to 5-ALA PDT in vitro, malignant MT-ret melanomas in vivo are quite resistant to this type of therapy at doses which are highly effective in vitro. |
| تدمد: | 1600-0625 0906-6705 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f55deea8ce40d507d5f332c219f144d https://doi.org/10.1111/j.0906-6705.2005.00303.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6f55deea8ce40d507d5f332c219f144d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000625 09066705 |
|---|