Simultaneous quantitation of three major triterpenoid glycosides inCentella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection
| العنوان: | Simultaneous quantitation of three major triterpenoid glycosides inCentella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection |
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| المؤلفون: | Jia Zhu, Ying-Jie Wei, Feng-Lun Zhang, Zhu-Nan Gong |
| المصدر: | Biomedical Chromatography. 22:119-124 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Light, Calibration curve, Clinical Biochemistry, Analytical chemistry, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, Centella, chemistry.chemical_compound, Chromatography detector, Drug Discovery, Trifluoroacetic acid, Scattering, Radiation, Glycosides, Molecular Biology, Chromatography, High Pressure Liquid, Pharmacology, chemistry.chemical_classification, Chromatography, biology, Plant Extracts, Elution, Reproducibility of Results, Glycoside, General Medicine, Repeatability, Saponins, biology.organism_classification, Triterpenes, chemistry |
| الوصف: | A high-performance liquid chromatography method with evaporative light scattering detection was established for simultaneous determination of three major triterpenoid glycosides, i.e. asiaticoside, madecassoside and asiaticoside-B, in Centella asiatica extracts. The optimal chromatographic conditions were achieved on a COSMOSIL 5C(18)-MS-II column by constant elution with water (0.01% trifluoroacetic acid, v/v) and acetonitrile (1.0% methyl tert-butyl ether, 0.01% trifluoroacetic acid, v/v) (78:22) as mobile phase at a flow rate of 1.0 mL/min; the column temperature was 30 degrees C. The evaporative light scattering detector was set at an evaporating temperature of 40 degrees C and nitrogen gas pressure of 3.5 bar. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability and accuracy. All calibration curves showed good linear regression (r(2) > 0.9993) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 1.73-3.06 and 3.89%-4.92%, respectively, and overall recoveries of 97.63-99.39% for the three compounds analyzed. The method developed was successfully applied to quantify the main triterpenoid glycosides in Centella asiatica extracts from different companies. |
| تدمد: | 1099-0801 0269-3879 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f77110b6269b399a59db09a0fa048f7 https://doi.org/10.1002/bmc.901 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6f77110b6269b399a59db09a0fa048f7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990801 02693879 |
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