A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae
| العنوان: | A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae |
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| المؤلفون: | Gregory A. Tucker, Chenyu Du, Ethiraju R. Keerthiraju, Darren Greetham |
| المصدر: | Microorganisms Volume 7 Issue 11 Microorganisms, Vol 7, Iss 11, p 575 (2019) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Microbiology (medical), Programmed cell death, COX20, Saccharomyces cerevisiae, yeast, medicine.disease_cause, Microbiology, Article, 03 medical and health sciences, Virology, medicine, Cytochrome c oxidase, oxidative stress, lcsh:QH301-705.5, programmed cell death, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, biology, 030306 microbiology, respiratory growth, biology.organism_classification, Yeast, 3. Good health, Cell biology, lcsh:Biology (General), chemistry, Apoptosis, biology.protein, Fermentation, Oxidative stress |
| الوصف: | Industrial production of bioethanol from lignocellulosic materials (LCM&prime s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance to acetic acid, a common inhibitory compound produced during pre-treatment of LCM&rsquo s. The presence of acetic acid has been shown to induce oxidative stress and programmed cell death, so the role of COX20 in oxidative stress was determined. Analysis using flow cytometry revealed that COX20 expression was associated with reduced levels of reactive oxygen species (ROS) in hydrogen peroxide and metal-induced stress, and there was a reduction in apoptotic and necrotic cells when compared with a strain without COX20. Results on the functionality of COX20 have revealed that overexpression of COX20 induced respiratory growth in &Delta imp1 and &Delta cox18, two genes whose presence is essential for yeast respiratory growth. COX20 also has a role in protecting the yeast cell against programmed cell death. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-2607 |
| DOI: | 10.3390/microorganisms7110575 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f989a2d2921907c238d06756b4c4d80 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6f989a2d2921907c238d06756b4c4d80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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| DOI: | 10.3390/microorganisms7110575 |