Analysis of a gene region required for dihydrogen oxidation in Azotobacter vinelandii
| العنوان: | Analysis of a gene region required for dihydrogen oxidation in Azotobacter vinelandii |
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| المؤلفون: | Jack Chien Chen, L. E. Mortenson, Lance C. Seefeldt |
| المصدر: | Current Microbiology. 30:351-355 |
| بيانات النشر: | Springer Science and Business Media LLC, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Genetics, Azotobacter vinelandii, Hydrogenase, biology, Operon, Mutant, General Medicine, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Open Reading Frames, Open reading frame, Biochemistry, Genes, Bacterial, Nickel, Mutation, ORFS, Oxidation-Reduction, Gene, Hydrogen, Azotobacteraceae |
| الوصف: | The putative products of six Azotobacter vinelandii chromosomal open reading frames (ORFs) were suggested to be involved in dihydrogen (H2) metabolism [Chen and Mortenson (1992) Biochim Biophys Acta 1131, 199-202]. A promoterless lacZ-containing cassette was used to disrupt the ORFs. Qualitative analysis revealed that the lacZ genes were expressed only in those mutants where the directions of the inserted lacZ were identical to those of the ORFs, showing that the six ORFs were transcribed as predicted. Unlike wildtype (w.t.), none of the mutants could perform dioxygen (O2)-dependent H2-oxidation, even though Western immunoanalyses showed that the hydrogenase large subunit was present although in amounts less than in w.t. Only one of the mutants (a hypB mutant), grown in nickel-enriched media, showed meaningful restoration of the H2-oxidizing ability. From the above observations it is concluded that (a) the six-ORF region is transcriptionally active and involved in H2-oxidation, (b) the product of hypB is needed for nickel activation of hydrogenase, and (c) the six ORFs (genes) belong to two or more operons. Possible roles of the gene products for the assembly, modification, and processing of hydrogenase from its apoproteins and metal centers are discussed. |
| تدمد: | 1432-0991 0343-8651 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fa4f8112301107f9119419ccf7bc47f https://doi.org/10.1007/bf00369862 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6fa4f8112301107f9119419ccf7bc47f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320991 03438651 |
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