Improving the detection of rare native fish species in environmental DNA metabarcoding surveys
| العنوان: | Improving the detection of rare native fish species in environmental DNA metabarcoding surveys |
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| المؤلفون: | Jack Rojahn, Dianne Gleeson, Jonas Bylemans, Tim Haeusler, Elise M. Furlan |
| المصدر: | Aquatic Conservation: Marine and Freshwater Ecosystems ARPHA Conference Abstracts 4: e65387 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | blocking primer, Ecology, Ecology (disciplines), General Engineering, Biodiversity, Fish species, Zoology, Aquatic Science, Biology, environmental DNA, fishes, metabarcoding, Aquatic science, Nature and Landscape Conservation, Environmental DNA, Alien species |
| الوصف: | The presence of threatened or endangered species often strongly influences management and conservation decisions. Within the Murray–Darling Basin (MDB), Australia, the presence of threatened native fish affects the management and allocation of water resources. These decisions are currently based on traditional fisheries data and a predictive MaxEnt model. However, it is important to verify the model's predictive power given the implication it may have, but this requires methods with a high detection sensitivity for rare species. Although the use of environmental DNA (eDNA) metabarcoding achieves a higher detection sensitivity compared with traditional methods, earlier surveys in the MDB have shown that the highly abundant and invasive common carp (Cyprinus carpio) can reduce detection probabilities for rare species. Consequently, a polymerase chain reaction (PCR) blocking primer designed to block the amplification of carp eDNA could increase the detection probabilities for rare native species while simultaneously reducing the required sampling effort and survey costs. Although PCR blocking primers are often used in ancient DNA and dietary studies, no aquatic eDNA metabarcoding study to date has evaluated the potential benefits of using PCR blocking primers. A laboratory and field‐based pilot study was used to address this knowledge gap and assess the impact of a blocking primer on the detection probabilities of native species and the minimum sampling effort required. Results showed that the inclusion of the blocking primer increased the detection probabilities for native species by 10–20% and reduced the minimum required sampling effort by 25–50%. These findings provide important insights into possible methods for optimizing eDNA metabarcoding surveys for the detection of rare aquatic species. |
| وصف الملف: | application/pdf; text/html |
| تدمد: | 1099-0755 1052-7613 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70110d686ffbfd3dc84f197080895e87 https://doi.org/10.1002/aqc.3514 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....70110d686ffbfd3dc84f197080895e87 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990755 10527613 |
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