We present an inventory of Iridaceae species from Serra dos Pireneus, a mountain range with several distinct vegetation types within the Cerrado biome and situated in the mid-east region of Goiás state, Brazil. The samples were collected in situ and surveys of herbaria were conducted. We found nine species of Iridaceae on Serra dos Pireneus, belonging to the following genera: Cipura, Gelasine, Sisyrinchium, Sphenostigma and Trimezia. This study provides a list of all species of Iridaceae recorded in this area including a brief diagnosis, a taxonomic note on Sphenostigma polycephalum, an identification key and an illustrated guide of Iridaceae.
