Statistical Laws of Protein Motion in Neuronal Dendritic Trees
| العنوان: | Statistical Laws of Protein Motion in Neuronal Dendritic Trees |
|---|---|
| المؤلفون: | Erin M. Schuman, Fabio Sartori, Tatjana Tchumatchenko, Yombe Fonkeu, Ali Karimi, Andreas Nold, Anne-Sophie Hafner |
| المصدر: | Cell Rep Cell Rep. Cell Reports, Vol 33, Iss 7, Pp 108391-(2020) Cell Reports |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, 3d electron microscopy, Models, Neurological, Primary Cell Culture, Kinesins, Rall’s rule, Plasticity, Article, General Biochemistry, Genetics and Molecular Biology, Rats, Sprague-Dawley, Mice, 03 medical and health sciences, 0302 clinical medicine, branching radii, medicine, Animals, radii optimization, lcsh:QH301-705.5, Total protein, Neurons, Models, Statistical, Neuronal Plasticity, Cell plasma membrane, Chemistry, 3D EM neuronal reconstructions, Protein species, Dyneins, intracellular diffusion, Dendrites, dendritic morphology, Rats, Mice, Inbred C57BL, Synaptic function, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), Cytoplasm, Synapses, Biophysics, Female, Neuron, 030217 neurology & neurosurgery |
| الوصف: | Summary Across their dendritic trees, neurons distribute thousands of protein species that are necessary for maintaining synaptic function and plasticity and that need to be produced continuously and trafficked to their final destination. As each dendritic branchpoint splits the protein flow, increasing branchpoints decreases the total protein number downstream. Consequently, a neuron needs to produce more proteins to maintain a minimal protein number at distal synapses. Combining in vitro experiments and a theoretical framework, we show that proteins that diffuse within the cell plasma membrane are, on average, 35% more effective at reaching downstream locations than proteins that diffuse in the cytoplasm. This advantage emerges from a bias for forward motion at branchpoints when proteins diffuse within the plasma membrane. Using 3D electron microscopy (EM) data, we show that pyramidal branching statistics and the diffusion lengths of common proteins fall into a region that minimizes the overall protein need. Graphical Abstract Highlights • Surface proteins are more efficient at reaching distal sites than soluble proteins • Daughter radius optimization reduces the number of proteins needed to populate dendrites • Ratios of daughter radii at branchpoints are cell type specific • Highly diffusive proteins incur a smaller extra cost for non-optimized radii Sartori et al. show that surface diffusion is more effective at providing proteins for distal dendritic sites than cytoplasmic diffusion. Daughter radius distribution at branchpoints can be optimized for long-range protein transport and reduce the total protein count necessary to populate distal synapses by orders of magnitude. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70b66d36808e9889a16cf648311a45b5 https://hdl.handle.net/21.11116/0000-0007-EEE3-D |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....70b66d36808e9889a16cf648311a45b5 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |