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Enhancement of recombinant human IL-24 (rhIL-24) protein production from site-specific integrated engineered CHO cells by sodium butyrate treatment

التفاصيل البيبلوغرافية
العنوان: Enhancement of recombinant human IL-24 (rhIL-24) protein production from site-specific integrated engineered CHO cells by sodium butyrate treatment
المؤلفون: Jilei, Hua, Hanli, Xu, Yao, Zhang, Jianlin, Ge, Mengzhe, Liu, Yuqi, Wang, Yuexian, Wei, Yinan, Shi, LingLing, Hou, Hong, Jiang
المصدر: Bioprocess and Biosystems Engineering. 45:1979-1991
بيانات النشر: Springer Science and Business Media LLC, 2022.
سنة النشر: 2022
مصطلحات موضوعية: Butyrates, Cricetulus, Cricetinae, Interleukins, Animals, Humans, Butyric Acid, Bioengineering, CHO Cells, General Medicine, Biotechnology
الوصف: Interleukin-24 (IL-24) has specific inhibitory effects on the proliferation of various tumor cells with almost no toxicity to normal cells. The antitumor activity of recombinant human IL-24 protein produced in mammalian cells is much higher than that of bacteria, but its expression level is extremely low. Sodium butyrate (NaBu) was utilized as a media additive to increase protein expression in Chinese hamster ovary cells. The site-specific integrated engineered cells FCHO/IL-24 were treated with NaBu under different culture conditions (10% and 0.5% serum adherent culture, 0.5% serum suspension culture). First, 3 days of 1 mmol/L NaBu treatment significantly increased rhIL-24 expression level in FCHO/IL-24 cells by 119.94 ± 1.5% (**p p p p p p p
تدمد: 1615-7605
1615-7591
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7103b95ce80ae0727557500cecaa7f18
https://doi.org/10.1007/s00449-022-02801-0
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....7103b95ce80ae0727557500cecaa7f18
قاعدة البيانات: OpenAIRE
الوصف
تدمد:16157605
16157591