Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis
| العنوان: | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
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| المؤلفون: | Barbara Danielewska-Nikiel, Nobuo Okado, Fukutaro Mizuhashi, Christina Sulaiman, Mai Sugi, Sa Pham, Ashley Roberts, Sawako Kasamoto |
| المصدر: | Food Science & Nutrition Food Science & Nutrition, Vol 8, Iss 1, Pp 456-478 (2020) Food Science & Nutrition, 8(1):456-478 |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | subchronic toxicity, 040301 veterinary sciences, lcsh:TX341-641, genotoxicity, food safety, Arabinase, Aspergillus tubingensis, Pharmacology, medicine.disease_cause, Chromosome aberration, Ames test, 0403 veterinary science, 0404 agricultural biotechnology, In vivo, Oral administration, medicine, Original Research, Chemistry, 04 agricultural and veterinary sciences, 040401 food science, In vitro, Micronucleus, lcsh:Nutrition. Foods and food supply, Genotoxicity, Food Science |
| الوصف: | Arabinase is an enzyme recognized for its ability to degrade arabinan, a plant cell wall constituent. It has been applied in the food industry most commonly for juice processing. One commercial source of arabinase is Aspergillus tubingensis (A. tubingensis), a black Aspergillus species. Given the intended use in food for human consumption, and noting its potential presence at trace levels in finished products, a series of safety studies including in vitro Ames and chromosome aberration assays, in vivo mammalian erythrocyte micronucleus and alkaline comet assays, and a 90‐day rat oral toxicity study were conducted. No test article‐related mutagenic activity was observed in the Ames assay. Although positive activity was observed in the chromosome aberration assay, this was not replicated in the in vivo genotoxicity assays including in preabsorptive cells. In the subchronic toxicity study, no test article‐related adverse effects were observed following oral administration of arabinase at doses of 15.3, 153, or 1,530 mg total organic solids (TOS)/kg body weight/day to Sprague Dawley rats. The no‐observed‐adverse‐effect level was considered to be the highest dose tested (1,530 mg TOS/kg body weight/day). The results of the genotoxicity studies and the subchronic toxicity study support the safe use of arabinase from A. tubingensis in food production. A series of toxicological studies including in vitro and in vivo short‐term genotoxicity assays and a 90‐day subchronic oral toxicity study were conducted to evaluate the safety of arabinase from Aspergillus tubingensis. Arabinase was concluded to be nongenotoxic and was not associated with toxicity in rats at the highest dose tested (up to 1,530 mg TOS/kg body weight/day). Study results provide reassurance of the safety of use of arabinase from A. tubingensis in food production. |
| اللغة: | English |
| تدمد: | 2048-7177 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7186a1e95670c6397dd2d77904cf3711 http://europepmc.org/articles/PMC6977434 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7186a1e95670c6397dd2d77904cf3711 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20487177 |
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