LPS-Enhanced Glucose-Stimulated Insulin Secretion Is Normalized by Resveratrol
| العنوان: | LPS-Enhanced Glucose-Stimulated Insulin Secretion Is Normalized by Resveratrol |
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| المؤلفون: | Anete Dudele, Bjørn Richelsen, Lars Porskjær Christensen, Sten Lund, Morten Poulsen, Mark K. Nøhr, Niels Jessen, Yulia Radko, Steen B. Pedersen, Lene Hyldahl Ebbesen |
| المصدر: | Nøhr, M K, Dudele, A, Poulsen, M M, Ebbesen, L H, Radko, Y, Christensen, L P, Jessen, N, Richelsen, B, Lund, S & Pedersen, S B 2016, ' LPS-Enhanced Glucose-Stimulated Insulin Secretion Is Normalized by Resveratrol ', PLOS ONE, vol. 11, no. 1, e0146840 . https://doi.org/10.1371/journal.pone.0146840 Nøhr, M K, Dudele, A, Poulsen, M M, Ebbesen, L H, Radko, Y, Christensen, L P, Jessen, N, Richelsen, B, Lund, S & Pedersen, S B 2016, ' LPS-enhanced glucose-stimulated insulin secretion is normalized by resveratrol ', P L o S One, vol. 11, no. 1, e0146840 . https://doi.org/10.1371/journal.pone.0146840 PLoS ONE PLoS ONE, Vol 11, Iss 1, p e0146840 (2016) |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Blood Glucose, Lipopolysaccharides, Male, 0301 basic medicine, Osmosis, Glucose/chemistry, medicine.medical_treatment, lcsh:Medicine, Adipose tissue, Resveratrol, Antioxidants, Mice, chemistry.chemical_compound, 0302 clinical medicine, Stilbenes, Insulin Secretion, Leukocytes, Insulin, Homeostasis, Glucose homeostasis, lcsh:Science, Epididymis, Glucose tolerance test, Multidisciplinary, medicine.diagnostic_test, Adipose Tissue/pathology, 3. Good health, Adipose Tissue, Liver, Liver/pathology, medicine.symptom, Research Article, medicine.medical_specialty, Leukocytes/cytology, Insulin/metabolism, Obesity/drug therapy, 030209 endocrinology & metabolism, Inflammation, Biology, Stilbenes/chemistry, 03 medical and health sciences, Insulin resistance, Internal medicine, medicine, Animals, Obesity, Adiponectin, Gene Expression Profiling, lcsh:R, Body Weight, Antioxidants/chemistry, Blood Glucose/analysis, Glucose Tolerance Test, medicine.disease, Mice, Inbred C57BL, Glucose, 030104 developmental biology, Endocrinology, chemistry, lcsh:Q, Insulin Resistance, Lipopolysaccharides/chemistry |
| الوصف: | Low-grade inflammation is seen with obesity and is suggested to be a mediator of insulin resistance. The eliciting factor of low-grade inflammation is unknown but increased permeability of gut bacteria-derived lipopolysaccharides (LPS) resulting in endotoxemia could be a candidate. Here we test the effect of LPS and the anti-inflammatory compound resveratrol on glucose homeostasis, insulin levels and inflammation. Mice were subcutaneously implanted with osmotic mini pumps infusing either low-dose LPS or saline for 28 days. Half of the mice were treated with resveratrol delivered through the diet. LPS caused increased inflammation of the liver and adipose tissue (epididymal and subcutaneous) together with enlarged spleens and increased number of leukocytes in the blood. Resveratrol specifically reduced the inflammatory status in epididymal fat (reduced expression of TNFa and Il1b, whereas the increased macrophage infiltration was unaltered) without affecting the other tissues investigated. By LC-MS, we were able to quantitate resveratrol metabolites in epididymal but not subcutaneous adipose tissue. LPS induced insulin resistance as the glucosestimulated insulin secretion during an oral glucose tolerance test was increased despite similar plasma glucose level resulting in an increase in the insulinogenic index (IGI; delta 0-15insulin/delta 0-15glucose) from 13.73 to 22.40 pmol/mmol (P < 0.001). This aberration in insulin and glucose homeostasis was normalized by resveratrol. In conclusion: Low-dose LPS enhanced the glucose-stimulated insulin secretion without affecting the blood glucose suggesting increased insulin resistance. Resveratrol restored LPS-induced alteration of the insulin secretion and demonstrated anti-inflammatory effects specifically in epididymal adipose tissue possibly due to preferential accumulation of resveratrol metabolites pointing towards a possible important involvement of this tissue for the effects on insulin resistance and insulin secretion. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::718879a591046c7603e7e29f8f2f9132 https://doi.org/10.1371/journal.pone.0146840 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....718879a591046c7603e7e29f8f2f9132 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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