The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures
| العنوان: | The role of detoxification enzymes in the susceptibility of Brevipalpus californicus exposed to acaricide and insecticide mixtures |
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| المؤلفون: | Thomas Van Leeuwen, Jaqueline Franciosi Della Vechia, Guilherme Duarte Rossi, Daniel Júnior de Andrade |
| المساهمون: | Universidade Estadual Paulista (Unesp), Ghent University |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Insecticides, Health, Toxicology and Mutagenesis, Tenuipalpidae, 01 natural sciences, Toxicology, Insecticide Resistance, 03 medical and health sciences, chemistry.chemical_compound, Imidacloprid, parasitic diseases, Mite, Animals, GST, Pesticides, Acaricides, Antagonism, Mites, Brevipalpus, biology, Acaricide, Esterases, Synergism, General Medicine, Phosmet, Pesticide, Monooxygenases, biology.organism_classification, 010602 entomology, 030104 developmental biology, Metabolism, chemistry, Agronomy and Crop Science |
| الوصف: | Made available in DSpace on 2021-06-25T10:28:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The intense spraying of pesticides to control different arthropod pests has resulted in negative side effects for the management of pests. It was previously discovered that exposure to non-acaricidal insecticides alone or in a mixture, results in lower efficiency of the acaricide spirodiclofen used for Brevipalpus spp. control. We investigate here whether the induced expression of detoxification enzymes by non-lethal insecticides may antagonize spirodiclofen toxicity. Brevipalpus californicus mites exposed to the insecticide phosmet alone or in combination with spirodiclofen showed increased activity of P450 monooxygenases (P450s). No antagonistic effects in mite mortality were observed by the combination of phosmet and spirodiclofen. On the other hand, mites exposed to the insecticide imidacloprid alone or in combination with spirodiclofen showed an increase in the activity of P450s, carboxylcholinesterases (CCE), and glutathione-S-transferases (GST). An antagonistic effect on mite mortality was observed when mites were exposed to the LC25 of spirodiclofen combined with the field rate treatment of imidacloprid. The addition of PBO (a P450 monooxygenase inhibitor) to the mixture of spirodiclofen and imidacloprid resulted in a synergistic effect over mite mortality but the addition of DEM (a GST inhibitor) resulted in an antagonist effect. Taken together, this study showed that the combination of imidacloprid with spirodiclofen is antagonistic for the control of B. californicus, and this results from the induction of detoxification enzymes, such as P450s, CCE, and GST. The use of inhibitors highlights the role of these enzymes in the antagonism of the mixture. Department of Agricultural Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane S/N. Zip code: 14 Laboratory of Agrozoology Department of Crop Protection Faculty of Bioscience Engineering Ghent University, Coupure Links 653 Department of Agricultural Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane S/N. Zip code: 14 FAPESP: #2019/08384-9 FAPESP: #301492/2018-2 |
| تدمد: | 1095-9939 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71a93f436ad52a1da6f18bb540787678 https://pubmed.ncbi.nlm.nih.gov/33993973 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....71a93f436ad52a1da6f18bb540787678 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959939 |
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