Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins
| العنوان: | Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins |
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| المؤلفون: | Marco Milanese, August B. Smit, Giambattista Bonanno, Stéphane H. R. Oliet, Karen E. Carney, Mark H. G. Verheijen, Pim van Nierop, Ka Wan Li |
| المساهمون: | Molecular and Cellular Neurobiology, AIMMS, Neuroscience Campus Amsterdam - Brain Mechanisms in Health & Disease |
| المصدر: | Carney, K E, Milanese, M, van Nierop, P, Li, K W, Oliet, S H R, Smit, A B, Bonano, G & Verheijen, M H G 2014, ' Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins ', Journal of Proteome Research, vol. 13, no. 12, pp. 5918-5927 . https://doi.org/10.1021/pr500829z Journal of Proteome Research, 13(12), 5918-5927. American Chemical Society |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Proteomics, Proteome, Nerve Tissue Proteins, Biology, Biochemistry, Exocytosis, Mice, Tandem Mass Spectrometry, medicine, Animals, Cytoskeleton, Synaptosome, VAMP3, Brain, Membrane Proteins, General Chemistry, Cell biology, Mice, Inbred C57BL, Membrane glycoproteins, medicine.anatomical_structure, Gene Ontology, Membrane protein, Astrocytes, biology.protein, Astrocyte, Synaptosomes |
| الوصف: | Astrocytes are being increasingly recognized as crucial contributors to neuronal function at synapses, axons, and somas. Reliable methods that can provide insight into astrocyte proteins at the neuron-astrocyte functional interface are highly desirable. Here, we conducted a mass spectrometry analysis of Percoll gradient-isolated gliosomes, a viable preparation of glial subcellular particles often used to study mechanisms of astrocytic transmitter uptake and release and their regulation. Gliosomes were compared with synaptosomes, a preparation containing the neurotransmitter release machinery, and, accordingly, synaptosomes were enriched for proteins involved in synaptic vesicle-mediated transport. Interestingly, gliosome preparations were found to be enriched for different classes of known astrocyte proteins, such as VAMP3 (involved in astrocyte exocytosis), Ezrin (perisynaptic astrocyte cytoskeletal protein), and Basigin (astrocyte membrane glycoprotein), as well as for G-protein-mediated signaling proteins. Mass spectrometry data are available via ProteomeXchange with the identifier PXD001375. Together, these data provide the first detailed description of the gliosome proteome and show that gliosomes can be a useful preparation to study glial membrane proteins and associated processes. |
| تدمد: | 1535-3907 1535-3893 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7230bd7a2badd7eb4b69469aca73ffe9 https://pubmed.ncbi.nlm.nih.gov/25308431 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....7230bd7a2badd7eb4b69469aca73ffe9 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15353907 15353893 |
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