Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄 虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells
| العنوان: | Effects and mechanisms of the functional parts of Dahuang Zhechong Pill (大黄 虫丸) containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells |
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| المؤلفون: | Juntian Liu, Yuanyuan Ji, Peipei Lu, Na Liu |
| المصدر: | Chinese Journal of Integrative Medicine. 19:432-438 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Serum, medicine.medical_specialty, Protein Kinase C-alpha, Platelet-derived growth factor, Vascular smooth muscle, MAP Kinase Signaling System, medicine.medical_treatment, Myocytes, Smooth Muscle, Blood stasis, Pharmacology, Muscle, Smooth, Vascular, Rats, Sprague-Dawley, chemistry.chemical_compound, Cyclin D1, Internal medicine, Dahuang Zhechong pill, medicine, Animals, Pharmacology (medical), Extracellular Signal-Regulated MAP Kinases, Cell Proliferation, Platelet-Derived Growth Factor, biology, Growth factor, Cell Cycle, DNA, General Medicine, Cell cycle, Rats, Enzyme Activation, Endocrinology, Complementary and alternative medicine, chemistry, biology.protein, Cyclin-Dependent Kinase Inhibitor p27, Platelet-derived growth factor receptor, Drugs, Chinese Herbal |
| الوصف: | To investigate and compare the effects and mechanisms of three functional parts of Dahuang Zhechong Pill (DHZCP), including drugs with the function of removing blood stasis and promoting blood circulation (FP-I), drugs with the function of expelling heat and moistening dryness (FP-II), and drugs with the function of nourishing yin and replenishing blood (FP-III) of DHZCP, on platelet-derived growth factor (PDGF)-stimulated vascular smooth muscle cells (VSMCs) proliferation with the method of serum pharmacology.VSMCs proliferation of rat was assayed by measuring the cell viability with the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) method. DNA synthesis in VSMCs was examined by detecting 5'-bromo-2'-deoxyuridine incorporation with the immunocytochemical method. Cycle of VSMCs was evaluated with flow cytometry. Expression of cyclin D1, p27, PKCα, and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) was quantified by the Western blotting method.The FP-I and FP-III containing serum was capable of inhibiting PDGF-stimulated proliferation and DNA synthesis of VSMCs, arrested VSMCs in G phase, downregulated cyclin D1, and upregulated p27 expression (P0.01 or P0.05). The FP-I and FP-III containing serum also inhibited the PDGF-induced phosphorylation of tyrosine of ERK1/2 and PKCα expression (P0.01 or P0.05).FP-I and FP-III of DHZCP are able to inhibit VSMCs proliferation via interrupting PKCα-ERK1/2 signaling, modulating the expression of cell cycle proteins to result in arresting the cells in G phase. The inhibitory effect is mainly related to the function of removing blood stasis and promoting blood circulation, slightly to the function of nourishing yin and replenishing blood, but not to the function of expelling heat and moistening dryness. |
| تدمد: | 1993-0402 1672-0415 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72390bd13398c3ce9ba33a563ac715d8 https://doi.org/10.1007/s11655-012-1033-y |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....72390bd13398c3ce9ba33a563ac715d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19930402 16720415 |
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