In vivo gene expression profiling of the entomopathogenic fungus Beauveria bassiana elucidates its infection stratagems in Anopheles mosquito
| العنوان: | In vivo gene expression profiling of the entomopathogenic fungus Beauveria bassiana elucidates its infection stratagems in Anopheles mosquito |
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| المؤلفون: | Huan Chen, Yiling Lai, Sibao Wang, Fang Li, Guandong Wang, Ge Wei |
| المصدر: | Science China Life Sciences. 60:839-851 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Hyphal growth, biology, Effector, Gene Expression Profiling, fungi, 030106 microbiology, Beauveria bassiana, Bassiana, Fungus, biology.organism_classification, Antioxidants, General Biochemistry, Genetics and Molecular Biology, Microbiology, 03 medical and health sciences, Anopheles, Host-Pathogen Interactions, Entomopathogenic fungus, Animals, Beauveria, General Agricultural and Biological Sciences, Anopheles stephensi, Signal Transduction, General Environmental Science |
| الوصف: | The use of entomopathogenic fungi to control mosquitoes is a promising tool for reducing vector-borne disease transmission. To better understand infection stratagems of insect pathogenic fungi, we analyzed the global gene expression profiling of Beauveria bassiana at 36, 60, 84 and 108 h after topical infection of Anopheles stephensi adult mosquitoes using RNA sequencing (RNA-Seq). A total of 5,354 differentially expressed genes (DEGs) are identified over the course of fungal infection. When the fungus grows on the mosquito cuticle, up-regulated DEGs include adhesion-related genes involved in cuticle attachment, Pth11-like GPCRs hypothesized to be involved in host recognition, and extracellular enzymes involved in the degradation and penetration of the mosquito cuticle. Once in the mosquito hemocoel, the fungus evades mosquito immune system probably through up-regulating expression of β-1,3-glucan degrading enzymes and chitin synthesis enzymes for remodeling of cell walls. Moreover, six previous unknown SSCP (small secreted cysteine-rich proteins) are significantly up-regulated, which may serve as "effectors" to suppress host defense responses. B. bassiana also induces large amounts of antioxidant genes to mitigate host-generated exogenous oxidative stress. At late stage of infection, B. bassiana activates a broad spectrum of genes including nutrient degrading enzymes, some transporters and metabolism pathway components, to exploit mosquito tissues and hemolymph as a nutrient source for hyphal growth. These findings establish an important framework of knowledge for further comprehensive elucidation of fungal pathogenesis and molecular mechanism of Beauveria-mosquito interactions. |
| تدمد: | 1869-1889 1674-7305 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::729e95b2f8ef953466236a1acb0e6f75 https://doi.org/10.1007/s11427-017-9101-3 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....729e95b2f8ef953466236a1acb0e6f75 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 18691889 16747305 |
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