Critical amino acid residues of maurocalcine involved in pharmacology, lipid interaction and cell penetration
| العنوان: | Critical amino acid residues of maurocalcine involved in pharmacology, lipid interaction and cell penetration |
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| المؤلفون: | Flavie Strappazzon, Rémy Sadoul, Hervé Darbon, Michel De Waard, Kamel Mabrouk, Amel Rehaim, Michel Ronjat, Sylvie Boisseau, Narendra Ram |
| المساهمون: | Ingénierie des protéines (IP), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Canaux calciques , fonctions et pathologies, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurodegenerescence et Plasticite, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Biochimie et Biologie Moléculaire, Faculté des Sciences de Tunis, Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Inserm, CEA, UJF, Région Rhône-Alpes, Collaboration, Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM)-Université de Tunis El Manar (UTM), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Canepari, Marco |
| المصدر: | Biochimica et Biophysica Acta-Molecular Cell Research Biochimica et Biophysica Acta-Molecular Cell Research, Elsevier, 2007, 1768 (10), pp.2528-40. ⟨10.1016/j.bbamem.2007.06.030⟩ Biochimica et Biophysica Acta-Molecular Cell Research, 2007, 1768 (10), pp.2528-40. ⟨10.1016/j.bbamem.2007.06.030⟩ |
| بيانات النشر: | Elsevier B.V. |
| مصطلحات موضوعية: | Cell Survival, Molecular Sequence Data, MESH: Ryanodine, Biophysics, Scorpion Venoms, MESH: Cricetinae, Peptide, CHO Cells, MESH: Amino Acid Sequence, Biology, Biochemistry, Membrane Lipids, Structure-Activity Relationship, 03 medical and health sciences, Cricetulus, MESH: Structure-Activity Relationship, MESH: Scorpion Venoms, MESH: Cricetulus, MESH: CHO Cells, Cricetinae, Animals, Structure–activity relationship, MESH: Animals, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Amino Acid Sequence, Peptide sequence, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Cell penetrating peptide, MESH: Molecular Sequence Data, Lipid interaction, Ryanodine, Ryanodine receptor, Endoplasmic reticulum, 030302 biochemistry & molecular biology, Biological activity, Cell Biology, Cell toxicity, chemistry, MESH: Cell Survival, Maurocalcine, Cell-penetrating peptide, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Cell penetration, MESH: Membrane Lipids |
| الوصف: | International audience; Maurocalcine (MCa) is a 33-amino acid residue peptide that was initially identified in the Tunisian scorpion Scorpio maurus palmatus. This peptide triggers interest for three main reasons. First, it helps unravelling the mechanistic basis of Ca(2+) mobilization from the sarcoplasmic reticulum because of its sequence homology with a calcium channel domain involved in excitation-contraction coupling. Second, it shows potent pharmacological properties because of its ability to activate the ryanodine receptor. Finally, it is of technological value because of its ability to carry cell-impermeable compounds across the plasma membrane. Herein, we characterized the molecular determinants that underlie the pharmacological and cell-penetrating properties of maurocalcine. We identify several key amino acid residues of the peptide that will help the design of cell-penetrating analogues devoid of pharmacological activity and cell toxicity. Close examination of the determinants underlying cell penetration of maurocalcine reveals that basic amino acid residues are required for an interaction with negatively charged lipids of the plasma membrane. Maurocalcine analogues that penetrate better have also stronger interaction with negatively charged lipids. Conversely, less effective analogues present a diminished ability to interact with these lipids. These findings will also help the design of still more potent cell penetrating analogues of maurocalcine. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0005-2736 0167-4889 |
| DOI: | 10.1016/j.bbamem.2007.06.030 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72a013d08b25ba236b2b2701bf266a40 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....72a013d08b25ba236b2b2701bf266a40 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00052736 01674889 |
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| DOI: | 10.1016/j.bbamem.2007.06.030 |