Reagent for introducing pyrene residues in oligonucleotides
| العنوان: | Reagent for introducing pyrene residues in oligonucleotides |
|---|---|
| المؤلفون: | Vladimir A. Korshun, N. B. Pestov, K. R. Birikh, Yu. A. Berlin |
| المصدر: | Bioconjugate Chemistry. 3:559-562 |
| بيانات النشر: | American Chemical Society (ACS), 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Molecular Sequence Data, Oligonucleotides, Biomedical Engineering, Fluorescence spectrometry, Pharmaceutical Science, Bioengineering, Polymerase Chain Reaction, Chemical synthesis, chemistry.chemical_compound, Residue (chemistry), Organic chemistry, Pharmacology, Phosphoramidite, Pyrenes, Base Sequence, Oligonucleotide, Organic Chemistry, Combinatorial chemistry, Fluorescence spectra, Spectrometry, Fluorescence, chemistry, Reagent, Pyrene, Electrophoresis, Polyacrylamide Gel, Indicators and Reagents, Biotechnology |
| الوصف: | A novel pyrenyl-containing phosphoramidite reagent, N-[4-(1-pyrenyl)butyryl]-O1-(4,4'-dimethoxytrityl)-O2- [(diisopropylamino)(2-cyanoethoxy)phosphino]-3-amino-1 ,2-propanediol (5), has been synthesized from 4-(1-pyrenyl)butanoic acid in four steps with the 52% overall yield and used to incorporate pyrene residue(s) into oligonucleotides. Oligonucleotides 6 and 7, bearing one or two pyrenes at the 5'-terminus, have been prepared by means of that reagent, characterized with fluorescence spectra, and successfully used as primers in a polymerase chain reaction. |
| تدمد: | 1520-4812 1043-1802 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72e4084bcfd8d12e4400fbd3781fc10d https://doi.org/10.1021/bc00018a016 |
| رقم الأكسشن: | edsair.doi.dedup.....72e4084bcfd8d12e4400fbd3781fc10d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204812 10431802 |
|---|