TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression
| العنوان: | TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression |
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| المؤلفون: | Jiyoun Lee, Juhyun Kim, Hayoung Yang, Eun Mi Hwang, Jae Yong Park, Sungbo Shim, Seung Chan Kim, Jaewon Sim, Osung Kwon |
| المصدر: | Cells, Vol 10, Iss 2751, p 2751 (2021) Cells Volume 10 Issue 10 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Genetically modified mouse, Chromosomes, Artificial, Bacterial, Cerebellum, Kainic acid, cerebellum, QH301-705.5, Green Fluorescent Proteins, TWIK-1, Hippocampus, Mice, Transgenic, Article, Green fluorescent protein, chemistry.chemical_compound, Potassium Channels, Tandem Pore Domain, Gene expression, medicine, Animals, Entorhinal Cortex, dentate gyrus, Biology (General), Neurons, Kainic Acid, lateral entorhinal cortex, Chemistry, Dentate gyrus, bacterial artificial chromosome transgenic mouse, Reproducibility of Results, General Medicine, Entorhinal cortex, Up-Regulation, Cell biology, medicine.anatomical_structure, Models, Animal, Neuroglia |
| الوصف: | TWIK-1 is the first identified member of the two-pore domain potassium (K2P) channels that are involved in neuronal excitability and astrocytic passive conductance in the brain. Despite the physiological roles of TWIK-1, there is still a lack of information on the basic expression patterns of TWIK-1 proteins in the brain. Here, using a modified bacterial artificial chromosome (BAC), we generated a transgenic mouse (Tg mouse) line expressing green fluorescent protein (GFP) under the control of the TWIK-1 promoter (TWIK-1 BAC-GFP Tg mice). We confirmed that nearly all GFP-producing cells co-expressed endogenous TWIK-1 in the brain of TWIK-1 BAC-GFP Tg mice. GFP signals were highly expressed in various brain areas, including the dentate gyrus (DG), lateral entorhinal cortex (LEC), and cerebellum (Cb). In addition, we found that GFP signals were highly expressed in immature granule cells in the DG. Finally, our TWIK-1 BAC-GFP Tg mice mimic the upregulation of TWIK-1 mRNA expression in the hippocampus following the injection of kainic acid (KA). Our data clearly showed that TWIK-1 BAC-GFP Tg mice are a useful animal model for studying the mechanisms regulating TWIK-1 gene expression and the physiological roles of TWIK-1 channels in the brain. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72e464c35c6382717ffd1b666e409bdd https://doi.org/10.3390/cells10102751 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....72e464c35c6382717ffd1b666e409bdd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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