Next-Generation Sequencing (NGS)-Based Preimplantation Genetic Testing for Aneuploidy (PGT-A) of Trophectoderm Biopsy for Recurrent Implantation Failure (RIF) Patients: a Retrospective Study
| العنوان: | Next-Generation Sequencing (NGS)-Based Preimplantation Genetic Testing for Aneuploidy (PGT-A) of Trophectoderm Biopsy for Recurrent Implantation Failure (RIF) Patients: a Retrospective Study |
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| المؤلفون: | Jing Tong, Yichao Niu, Ting Zhang, Anran Wan |
| المصدر: | Reproductive Sciences |
| بيانات النشر: | Springer International Publishing, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, medicine.medical_specialty, medicine.medical_treatment, Aneuploidy, Fertilization in Vitro, Intracytoplasmic sperm injection, Miscarriage, Recurrent implantation failure, Pregnancy, Trophectoderm biopsy, medicine, Humans, Embryo Implantation, Genetic Testing, Preimplantation Diagnosis, Genetic testing, Retrospective Studies, Gynecology, In vitro fertilisation, medicine.diagnostic_test, business.industry, Preimplantation genetic testing for aneuploidy, Obstetrics and Gynecology, High-Throughput Nucleotide Sequencing, Retrospective cohort study, medicine.disease, Embryo transfer, Pregnancy rate, Blastocyst, Reproductive Genetics: Original Article, embryonic structures, Next-generation sequencing, Female, business, Infertility, Female |
| الوصف: | Recurrent implantation failure (RIF) is an intrigue condition during in vitro fertilization (IVF) cycles or intracytoplasmic sperm injection (ICSI) treatments. The purpose of this retrospective study is to explore the value of next-generation sequencing (NGS)-based preimplantation genetic testing for aneuploidy (PGT-A) of trophectoderm biopsy in the clinical outcomes for RIF patients with advanced age. A total of 265 RIF patients, who underwent 346 oocyte retrieval cycles and 250 PGT-A cycles, were classified as two groups according to the female age, including < 38 and ≥ 38 years old groups. The two groups were statistically comparable in baseline characteristics. The component of aneuploid embryos was significantly higher in advanced age group than in younger age group (68.9 vs 39.9%, P < 0.001). But there were no statistically significant differences in pregnancy rate (43.5 vs 64.7%), clinical pregnancy rate (39.1 vs 48.0%), implantation rate (39.1 vs 51.0%), and miscarriage rate (4.3 vs 7.8%) per embryo transfer (ET) between the two groups. Results suggest that the embryo-related factor plays a crucial role in RIF. Maternal age does not influence the implantation potential of euploid blastocysts. The NGS-based PGT-A involving trophectoderm biopsy is valuable for RIF patients of advanced age by improving their clinical outcomes. In conclusion, the NGS-based PGT-A involving trophectoderm biopsy may represent a valuable supplement to the current RIF management. Nonetheless, these findings should be further validated in a well-designed randomized controlled trial. |
| اللغة: | English |
| تدمد: | 1933-7205 1933-7191 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73466324947f9c704eb101ce0c50042a http://europepmc.org/articles/PMC8189974 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....73466324947f9c704eb101ce0c50042a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19337205 19337191 |
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