Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts
| العنوان: | Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts |
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| المؤلفون: | Kenneth L. Roy, Xiaoning Wu |
| المصدر: | Journal of Bacteriology. 175:37-52 |
| بيانات النشر: | American Society for Microbiology, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | DNA, Bacterial, Inverted repeat, Molecular Sequence Data, Restriction Mapping, Streptomyces clavuligerus, Sequence alignment, Microbiology, Open Reading Frames, Bacterial Proteins, Transcription (biology), Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Codon, Molecular Biology, Repetitive Sequences, Nucleic Acid, Genetics, Base Composition, Base Sequence, Sequence Homology, Amino Acid, biology, Nucleic acid sequence, RNA, Sequence Analysis, DNA, biology.organism_classification, Molecular biology, Streptomyces, Antisense RNA, Molecular Weight, Open reading frame, Nucleic Acid Conformation, Plasmids, Research Article |
| الوصف: | The complete nucleotide sequence of a small linear plasmid (pSCL1) from Streptomyces clavuligerus has been determined. This plasmid is 11,696 bp in length, has a 72% G+C content, and has approximately 900-bp inverted terminal repeat sequences. A comparison of the inverted terminal repeats of pSCL1 with those of a linear plasmid from S. rochei shows that the two terminal sequences have a high degree of similarity (approximately 70%). Several small inverted repeats found in the long terminal sequences of both plasmids are also conserved. An analysis of the sequence and codon preferences indicates that pSCL1 has seven or eight highly probable protein-coding open reading frames (ORFs). However, only two RNA species encoded by pSCL1 were detected in S. clavuligerus grown in liquid culture. The larger of these transcripts (900 nucleotides) corresponds to an ORF and is likely to be an mRNA for a protein similar to the KorA protein of pIJ101. The smaller transcript (460 nucleotides) does not correspond to any ORF; however, its 5' end is complementary to the 5' end of a predicted mRNA, suggesting that it may function as an antisense RNA. The larger of the two RNA species was present at a high level during the early stage of growth in liquid medium, and then its apparent rate of transcription decreased and remained at a lower level through the later stages; the level of the smaller RNA species remained relatively constant through all stages of growth. |
| تدمد: | 1098-5530 0021-9193 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::742f05b160d1797e92fbd2c1fce3ac5c https://doi.org/10.1128/jb.175.1.37-52.1993 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....742f05b160d1797e92fbd2c1fce3ac5c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985530 00219193 |
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