FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E‐cadherin and CD44 expression
| العنوان: | FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E‐cadherin and CD44 expression |
|---|---|
| المؤلفون: | Guoyan Liu, Yuling Hong, Kun Lu, Fan Liu, Xiao-Ling Deng, Li Yin, Haifeng Qin, Song-Lin Shi, Liu Lei, Li Long, Xunrong Zhuang, Yuhai Zhang |
| المصدر: | Journal of Cellular Physiology |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Physiology, Clinical Biochemistry, FNDC3B, migration, 03 medical and health sciences, Exon, 0302 clinical medicine, Circular RNA, Antigens, CD, Cell Movement, Stomach Neoplasms, Original Research Articles, Cell Line, Tumor, Humans, circRNA, Neoplasm Invasiveness, Original Research Article, RNA, Messenger, CD44, Messenger RNA, IGF2BP3, Expression vector, Base Sequence, Cadherin, Chemistry, RNA, RNA-Binding Proteins, Cell Biology, RNA, Circular, Cadherins, Molecular biology, Gene Expression Regulation, Neoplastic, 030104 developmental biology, Hyaluronan Receptors, 030220 oncology & carcinogenesis, RNA splicing, Ectopic expression |
| الوصف: | Circular RNAs (circRNAs) are a new class of RNAs, and many studies have identified thousands of circRNAs in tumor cells. Fibronectin type III domain‐containing protein 3B (FNDC3B) circular RNA (circFNDC3B, circBase ID: hsa_circ_0006156) circularizes with exons 5 and 6. Gibson Assembly DNA technology was used to construct a circFNDC3B expression vector without a splice site and restriction enzyme site. We showed that circFNDC3B increased migration and invasion in gastric cancer (GC). Ectopic expression of circFNDC3B reduced the level of E‐cadherin protein to promote the epithelial–mesenchymal transition in GC. RNA immunoprecipitation assays and RNA pull‐down assays confirmed that circFNC3B increased CD44 expression, which was associated with cell adhesion, via the formation of a ternary complex of circFNDC3B‐IGF2BP3‐CD44 mRNA. These results indicated that circFNDC3B was associated with the degree of malignancy to highlight the specific characteristics of cell invasion. |
| اللغة: | English |
| تدمد: | 1097-4652 0021-9541 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74e13b5175d656ffe1ef74566dd4b567 http://europepmc.org/articles/PMC6766960 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....74e13b5175d656ffe1ef74566dd4b567 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
|---|