Data on Connexin 43 hemichannels regulation of cellular redox in lens
| العنوان: | Data on Connexin 43 hemichannels regulation of cellular redox in lens |
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| المؤلفون: | Changrui Wu, Yumeng Quan, Sumin Gu, Yu Du, Jean X. Jiang |
| المصدر: | Data in Brief, Vol 39, Iss, Pp 107572-(2021) Data in Brief |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Science (General), Computer applications to medicine. Medical informatics, R858-859.7, Connexin, Oxidative phosphorylation, medicine.disease_cause, Hemichannel, Redox metabolite, chemistry.chemical_compound, Lens, Q1-390, Extracellular, medicine, Data Article, chemistry.chemical_classification, Reactive oxygen species, Multidisciplinary, Chemistry, Glutathione, Epithelium, Cell biology, medicine.anatomical_structure, Redox regulation, cardiovascular system, sense organs, biological phenomena, cell phenomena, and immunity, Intracellular, Oxidative stress |
| الوصف: | This article describes a dataset that is related to the research paper “Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage”. Growing evidence demonstrates that oxidative stress is a key event in cataract formation. Hemichannels (HCs) formed by Connexin (Cx) 43, a Cx subtype only present in the epithelium of lens tissue, mediate the exchange of small molecules between the intracellular and extracellular environments, including redox-related metabolic molecules, such as glutathione (GSH) and reactive oxygen species (ROS). Here, we used a Cx43 heterozygous mouse model, Cx43E2 antibody (a specific Cx43 HC blocker), and knocked down Cx43 expression by siRNA in human lens epithelial HLE-B3 cells to assess the oxidative response of Cx43 HCs to H2O2 and UVB radiation. Western blot analysis of heterozygous Cx43-null (Cx43+/−) mouse lenses showed the haploinsufficiency of Cx43 protein. We further assessed anti-oxidative gene expression in response to H2O2 and UVB radiation treatment in the Cx43-deficient lens epithelial cells. This dataset will be useful for understanding the critical role of Cx43 HCs in maintaining redox homeostasis in the lens under oxidative stress. |
| اللغة: | English |
| تدمد: | 2352-3409 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7601e8c6a58868d794980b7cd6dac449 http://www.sciencedirect.com/science/article/pii/S2352340921008477 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7601e8c6a58868d794980b7cd6dac449 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23523409 |
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