Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan
| العنوان: | Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan |
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| المؤلفون: | Bianca Nowlan, Satya P. Singh, M. S. Dodia, Bharat K. C. Patel |
| المصدر: | International Journal of Systematic and Evolutionary Microbiology |
| بيانات النشر: | Microbiology Society, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | DNA, Bacterial, Tetracycline, Movement, Molecular Sequence Data, India, Bacillus, Microbial Sensitivity Tests, Sodium Chloride, DNA, Ribosomal, Microbiology, Drug Resistance, Multiple, Bacterial, RNA, Ribosomal, 16S, medicine, Animalia, Alkaliphile, Bacillus (bacterium), Bacteria (microorganisms), Phylogeny, Soil Microbiology, Ecology, Evolution, Behavior and Systematics, Base Composition, Bacillaceae, biology, Temperature, Proteins, Genes, rRNA, Pigments, Biological, 060500 MICROBIOLOGY, General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, 16S ribosomal RNA, Bacillales, Aerobiosis, Growth Inhibitors, Bacillus krulwichiae, Anti-Bacterial Agents, Bacterial Typing Techniques, Spore, RNA, Bacterial, Posibacteria, Flagella, Halotolerance, Carbohydrate Metabolism, Bacteria, medicine.drug |
| الوصف: | A strictly aerobic, rod-shaped bacterium (0.6–0.8×2–3 μm), designated strain Kh10-101T, was isolated from a saltpan (22° 15′ N, 69° 1′ E) in the vicinity of Port Okha, India. The creamish pigmented colonies of strain Kh10-101T were round, flat and translucent with irregular margins and a smooth surface. The strain possessed up to three subpolar flagella, and was motile by a corkscrew motion. The strain grew optimally at 37 °C (temperature growth range 25–40 °C) in a complex glucose-containing medium with 5 % NaCl (NaCl growth range 0–10 %) at pH 9 (pH growth range pH 7–10), indicating that it was a mesophilic halotolerant alkaliphile. The strain was sensitive to lincomycin, meticillin, cefuroxime and cephalexin, but resistant to gentamicin, tetracycline and cotrimazine. Spores were not detected and cells were heat sensitive. The isolate metabolized a range of carbohydrates and hydrolysed casein, gelatin and starch. Growth was not observed on aromatic compounds, Tween 40 or Tween 80. Nitrate was not reduced and catalase was produced. Electron microscopic examination of thin sections revealed a single thick Gram-positive cell wall. The DNA G+C content was 41±1 mol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that strain Kh10-101T was a member of the sixth rRNA group of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The halotolerant obligate alkaliphile Bacillus krulwichiae is the closest relative of strain Kh10-101T (96 % similarity) but a number of phenotypic differences suggest that strain Kh10-101T (=JCM 13040T=ATCC BAA-1137T) should be designated the type strain of a new species, for which the name Bacillus okhensis sp. nov. is proposed. |
| تدمد: | 1466-5034 1466-5026 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76206d4866acd809598e872b6594aaed https://doi.org/10.1099/ijs.0.63861-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....76206d4866acd809598e872b6594aaed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14665034 14665026 |
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