A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain
| العنوان: | A spatially resolved brain region- and cell type-specific isoform atlas of the postnatal mouse brain |
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| المؤلفون: | Hagen Tilgner, Simon A. Hardwick, Geoffrey S. Pitt, Man Ying Wong, Andrey D. Prjibelski, Wenjie Luo, Ahmed Mahfouz, Stephen R. Williams, Zachary Bent, Susan Lin, Qi Wang, Adam Frankish, Alexander N. Stein, Bettina Haase, Paul Flicek, Olivier Fedrigo, Christoph Dieterich, Jordan Marrocco, Toby Hunt, Davide Risso, Erich D. Jarvis, August B. Smit, Paul Collier, Jennifer Chew, Steven A. Sloan, Ashley Hayes, Anoushka Joglekar, M. Elizabeth Ross, Neil I. Weisenfeld, Anna Katharina Schlusche |
| المساهمون: | Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, Amsterdam Neuroscience - Neurodegeneration |
| المصدر: | Nature Communications, 12(1) Nature Communications, 12(1). NATURE RESEARCH Joglekar, A, Prjibelski, A, Mahfouz, A, Collier, P, Lin, S, Schlusche, A K, Marrocco, J, Williams, S R, Haase, B, Hayes, A, Chew, J G, Weisenfeld, N I, Wong, M Y, Stein, A N, Hardwick, S A, Hunt, T, Wang, Q, Dieterich, C, Bent, Z, Fedrigo, O, Sloan, S A, Risso, D, Jarvis, E D, Flicek, P, Luo, W, Pitt, G S, Frankish, A, Smit, A B, Ross, M E & Tilgner, H U 2021, ' A spatially resolved brain region-and cell type-specific isoform atlas of the postnatal mouse brain ', Nature Communications, vol. 12, 463, pp. 1-16 . https://doi.org/10.1038/s41467-020-20343-5 Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021) Nature Communications Nature Communications, 12:463, 1-16. Nature Publishing Group |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Gene isoform, Cell type, RNA splicing, Science, General Physics and Astronomy, Prefrontal Cortex, Biology, Hippocampus, General Biochemistry, Genetics and Molecular Biology, Article, Transcriptome, 03 medical and health sciences, Exon, Mice, 0302 clinical medicine, Animals, Protein Isoforms, Prefrontal cortex, Transcriptomics, Gene, Regulation of gene expression, Spatial Analysis, Multidisciplinary, Computational Biology, Gene Expression Regulation, Developmental, Development of the nervous system, General Chemistry, Cell biology, Alternative Splicing, 030104 developmental biology, Animals, Newborn, Computational neuroscience, Models, Animal, Female, Single-Cell Analysis, 030217 neurology & neurosurgery |
| الوصف: | Splicing varies across brain regions, but the single-cell resolution of regional variation is unclear. We present a single-cell investigation of differential isoform expression (DIE) between brain regions using single-cell long-read sequencing in mouse hippocampus and prefrontal cortex in 45 cell types at postnatal day 7 (www.isoformAtlas.com). Isoform tests for DIE show better performance than exon tests. We detect hundreds of DIE events traceable to cell types, often corresponding to functionally distinct protein isoforms. Mostly, one cell type is responsible for brain-region specific DIE. However, for fewer genes, multiple cell types influence DIE. Thus, regional identity can, although rarely, override cell-type specificity. Cell types indigenous to one anatomic structure display distinctive DIE, e.g. the choroid plexus epithelium manifests distinct transcription-start-site usage. Spatial transcriptomics and long-read sequencing yield a spatially resolved splicing map. Our methods quantify isoform expression with cell-type and spatial resolution and it contributes to further our understanding of how the brain integrates molecular and cellular complexity. Alternative RNA splicing varies across the brain. Its mapping at single cell resolution is unclear. Here, the authors provide a spatial and single-cell splicing atlas reporting brain region- and cell type-specific expression of different isoforms in the postnatal mouse brain. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7712a28f4e2e541325f3f669a7004ac3 http://resolver.tudelft.nl/uuid:afc68c77-f8cf-451d-ae86-4172b8f59cd8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7712a28f4e2e541325f3f669a7004ac3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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