The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis
| العنوان: | The Effects of Vitamin D on the Expression of IL-33 and Its Receptor ST2 in Skin Cells; Potential Implication for Psoriasis |
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| المؤلفون: | Bogusław Nedoszytko, Justyna Wierzbicka, Joanna I. Nowak, Dorota Purzycka-Bohdan, Anna Olszewska, Roman Nowicki, Anna Piotrowska, Michał A. Żmijewski, Aneta Szczerkowska-Dobosz |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12907 International Journal of Molecular Sciences, Vol 22, Iss 12907, p 12907 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Keratinocytes, skin, Adolescent, QH301-705.5, vitamin D, IL-33, ST2, psoriasis, Calcitriol receptor, Catalysis, Article, Inorganic Chemistry, Immune system, Psoriasis, medicine, Vitamin D and neurology, Humans, Physical and Theoretical Chemistry, Biology (General), Receptor, Molecular Biology, QD1-999, Spectroscopy, Tissue homeostasis, Chemistry, Organic Chemistry, General Medicine, Vitamins, medicine.disease, Interleukin-33, Interleukin-1 Receptor-Like 1 Protein, Computer Science Applications, Interleukin 33, Case-Control Studies, Cancer research, Immunostaining |
| الوصف: | Interleukin 33 (IL-33) belongs to the IL-1 family and is produced constitutively by epithelial and endothelial cells of various organs, such as the skin. It takes part in the maintenance of tissue homeostasis, repair, and immune response, including activation of Th2 lymphocytes. Its involvement in pathogenesis of several inflammatory diseases including psoriasis was also suggested, but this is not fully understood. The aim of the study was to investigate expression of IL-33 and its receptor, ST2, in psoriasis, and the effects of the active form of vitamin D (1,25(OH)2D3) on their expression in skin cells. Here we examined mRNA and protein profiles of IL-33 and ST2 in 18 psoriatic patients and healthy volunteers by qPCR and immunostaining techniques. Potential effects of 1,25(OH)2D3 and its receptor (VDR) on the expression of IL-33 and ST2 were tested in cultured keratinocytes, melanocytes, fibroblasts, and basal cell carcinoma cells. It was shown that 1,25(OH)2D3 effectively stimulated expression of IL-33 and its receptor ST2’s mRNAs in a time-dependent manner, in keratinocytes and to the lesser extends in melanocytes, but not in fibroblasts. Furthermore, the effect of vitamin D on expression of IL-33 and ST2 was VDR-dependent. Finally, we demonstrated that the expression of mRNA for IL-33 was mainly elevated in the psoriatic skin but not in its margin. Interestingly, ST2 mRNA was downregulated in psoriatic lesion compared to both marginal tissue as well as healthy skin. Our data indicated that vitamin D can modulate IL-33 signaling, opening up new perspectives for our understanding of the mechanism of vitamin D action in psoriasis therapy. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7727786f8ed26ccec0292f923a7d31a1 http://europepmc.org/articles/PMC8657669 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7727786f8ed26ccec0292f923a7d31a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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