Strand-specific miR-28-3p and miR-28-5p have differential effects on nasopharyngeal cancer cells proliferation, apoptosis, migration and invasion
| العنوان: | Strand-specific miR-28-3p and miR-28-5p have differential effects on nasopharyngeal cancer cells proliferation, apoptosis, migration and invasion |
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| المؤلفون: | Xingkai Ma, Yan Lv, Geping Wu, Huijun Yang |
| المصدر: | Cancer Cell International, Vol 19, Iss 1, Pp 1-12 (2019) Cancer Cell International |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cancer Research, Nasopharyngeal cancer, Proliferation, Biology, medicine.disease_cause, lcsh:RC254-282, 03 medical and health sciences, miR-28-3p, 0302 clinical medicine, Cyclin D1, Invasion, microRNA, Genetics, medicine, otorhinolaryngologic diseases, miR-28-5p, lcsh:QH573-671, PI3K/AKT/mTOR pathway, Cell growth, Akt/PKB signaling pathway, lcsh:Cytology, Cell migration, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, stomatognathic diseases, Oncology, Nasopharyngeal carcinoma, 030220 oncology & carcinogenesis, Cancer research, Carcinogenesis, Primary Research |
| الوصف: | Background MicroRNAs (miRNAs) play crucial roles in varieties of cancers, particularly in tumorigenesis, progression, and migration. Dysregulation of miR-28 was reported to occur in various types of human malignancies. In humans, two different mature miRNA sequences are excised from opposite arms of the stem-loop pre-miR-28, hsa-miR-28-3p and hsamiR-28-5p. However, the expression and distinct role of miR-28-3p and miR-28-5p in nasopharyngeal carcinoma (NPC) remain undetermined. Methods The expressions of miR-28-3p/-5p in human NPC tissues were tested by quantitative real-time PCR. miR-28-3p/-5p were overexpressed by mimics and silenced by inhibitors. The roles of miR-28-3p/-5p in NPC development were studied using cultured HONE-1 cells. Results The mRNA expression levels of miR-28-3p and -5p were significantly decreased in NPC tissues in comparison with adjacent normal tissues. Overexpression of miR-28-5p suppressed NPC cell proliferation and induced cell cycle arrest and apoptosis, while miR-28-3p promoted NPC cell migration and invasion. The miRNAs effected on different signal pathways: miR-28-5p altered expression of cyclin D1 and influenced the PI3K/AKT signaling pathway. In contrast, miR-28-3p downregulated Nm23-H1 and accelerated the process of EMT. Conclusion miR-28-3p and -5p were both downregulated in NPC tissues but had distinct biological effects in NPC cells. They may serve as potential prognostic markers and therapeutic targets for NPC. |
| اللغة: | English |
| تدمد: | 1475-2867 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7729cabd44d852d45d8d4aa4afe93ad5 http://link.springer.com/article/10.1186/s12935-019-0915-x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7729cabd44d852d45d8d4aa4afe93ad5 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14752867 |
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