A Self‐Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High‐Resolution Structural Detail
| العنوان: | A Self‐Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High‐Resolution Structural Detail |
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| المؤلفون: | Tara MacCulloch, Chad R. Simmons, Yan Liu, Hao Yan, Nicholas Stephanopoulos, Fei Zhang |
| المصدر: | Angewandte Chemie. 132:18778-18785 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Materials science, 010405 organic chemistry, Base pair, Crystal system, General Chemistry, Crystal structure, General Medicine, 010402 general chemistry, 01 natural sciences, Catalysis, 0104 chemical sciences, Crystal, Crystallography, Sticky and blunt ends, Holliday junction, Molecular replacement, Self-assembly |
| الوصف: | DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 Å) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 Å) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 Å resolution by molecular replacement. |
| تدمد: | 1521-3757 0044-8249 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774a741c231e90ccecf18f6a855461ed https://doi.org/10.1002/ange.202005505 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....774a741c231e90ccecf18f6a855461ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15213757 00448249 |
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